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Medium Detail Information

Medium No.
1065 
Medium
Medium for strain JA363, JA395 and JA418 
Composition
 KH2PO40.5g
 MgSO4·7H2O2g
 NaCl20g
 NH4Cl0.6g
 CaCl2·2H2O0.15g
 Sodium pyruvate3g
 NaHCO3 (10% (w/v))10ml
 Yeast extract0.3g
 Peptone0.3g
 Ferric citrate solution* (0.1%)5ml
 Trace element solution SL7**1ml
 Na2S·9H2O0.24g
 Resazurin1mg
 Distilled water1L
 pH 8 
  Mix ingredients except for NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them.
       
  *Soluble in hot water.
       
  **Trace element solution SL7
   HCl (25%; 7.7 M)10ml
   FeCl2·4H2O1.5g
   ZnCl270mg
   MnCl2·4H2O100mg
   H3BO360mg
   CoCl2·6H2O200mg
   CuCl2·2H2O20mg
   NiCl2·6H2O20mg
   Na2MoO4·2H2O40mg
   Distilled water990ml
  Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.
 
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