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Medium Detail Information

Medium No.
1099
Medium
Modified NBRC medium 802 (pH 10, 0.6 M total Na+)
Composition
 Hipolypepton*10g
 Yeast extract2g
 MgSO4·7H2O1g
 K2HPO41g
 Distilled water900ml
 Agar (if needed)15g
       
  *Wako Pure Chemical Ind., Ltd., Osaka, Japan.
  After autoclaving, aseptically add 100 ml of a sodium carbonate buffer (for pH 10 and 0.6 M total Na+; 22% Na2CO3, 8% NaHCO3, 6% NaCl, 0.5% K2HPO4) to the medium.
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