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Medium Detail Information

Medium No.	1110
Medium	Alkalic MRS (pH 8.5) + 3% NaCl
Composition
Solution A* 900 ml   Solution B** 100 ml     Separately autoclave Solutions A and B. Mix aseptically. Final pH around 8.5.                   *Solution A       Peptone 10 g       Meat extract 10 g       Yeast extract 5 g       Glucose 20 g       Tween 80 1 g       K2HPO4 2 g       Sodium acetate 5 g       Diammonium hydrogen citrate 2 g       MgSO4·7H2O 0.2 g       MnSO4·nH2O 0.05 g       NaCl 30 g       Agar (if needed) 15 g       Distilled water 900 ml   pH unadjusted                     **Solution B       Na2CO3 6 g       Distilled water 100 ml   pH unadjusted
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