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Medium Detail Information

Medium No.	1194
Medium	Thiobacillus plumbophilus Medium
Composition
KCl 0.3 g   MgCl2·6H2O 2.75 g   MgSO4·7H2O 3.45 g   NH4Cl 1.25 g   CaCl2·2H2O 0.14 g   K2HPO4 0.14 g   NaCl 0.50 g   Trace element solution* 10 ml   NiCl2·6H2O 5 mg   Na2WO4·2H2O 0.5 mg   Distilled water 1 L     Dissolve mineral salts in water, dispense 20 ml amounts into 100 - 120 ml serum bottles under H2/CO2 (80/20) atmosphere, and autoclave. Before inoculation, add sterile air (final cocentration 3% v/v), and pressurize bottles to 150 kPa with H2/CO2.                   *Trace element solution       Nitrilotriacetic acid 1.5 g       MgSO4·7H2O 3 g       MnSO4·2H2O 0.5 g       NaCl 1 g       FeSO4·7H2O 0.1 g       CoSO4·7H2O 0.18 g       CaCl2·2H2O 0.1 g       ZnSO4·7H2O 0.18 g       CuSO4·5H2O 0.01 g       KAl(SO4)2·12H2O 0.02 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.01 g       NiCl2·6H2O 0.03 g       Na2SeO3·5H2O 0.3 mg       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
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