Medium No. |
1198 |
Medium |
DG18: Dichloran 18% Glycerol Agar |
Composition |
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| | Peptone | 5 | g | | | Glucose | 10 | g | | | KH2PO4 | 1 | g | | | MgSO4·7H2O | 0.5 | g | | | Dichloran (0.2% in ethanol) | 1.0 | ml | | | Glycerol | 220 | g | | | Chloramphenicol | 0.1 | g | | | Agar | 15.0 | g | | | Distilled water | 1 | L | | | | Add minor ingredients and agar to ca. 800 ml distilled water. Steam to dissolve agar, then make to one litre with distilled water. Add 220 g glycerol and sterilize by autoclaving at 121°C for 15 min. The final aw is 0.955, final pH 5.6±0.2. |
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Comment |
Commercial Dichloran glycerol agar base (Oxoid, MERCK, etc.) is also available. |