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Medium Detail Information

Medium No.	1224
Medium	Medium for Strain JA746
Composition
KH2PO4 0.5 g   MgSO4·7H2O 1 g   NaCl 20 g   NH4Cl 0.34 g   CaCl2·2H2O 0.25 g   Sodium pyruvate 2 g   Sodium succinate 2 g   Sodium acetate 2 g   Yeast extract 0.4 g   Ferric citrate solution* (0.1%) 5 ml   Trace element solution SL7** 1 ml   NaHCO3 solution (10% w/v) 15 ml   Na2S·9H2O 0.48 g   Vitamin B12 (0.002% (w/v)) 1 ml   Resazurin 1 mg   Distilled water 1 L   pH 7       Mix ingredients except vitamin B12, bicarbonate and sulfide, and dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers. After autoclaving (121°C, 15 min), aseptically and anaerobically add vitamin B12 solution, bicarbonate solution and sulfide solution using sterile syringes. The bicarbonate and vitamin solutions should be sterilized by filtration. Adjust the pH of the medium to pH 7.                   *Soluble in hot water.                   **Trace element solution SL7       HCl (25%; 7.7 M) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 60 mg       CoCl2·6H2O 200 mg       CuCl2·2H2O 20 mg       NiCl2·6H2O 20 mg       Na2MoO4·2H2O 40 mg       Distilled water 990 ml     Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.
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