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Medium Detail Information

Medium No.
1245 
Medium
Gluconacetobacter xylinus Medium 
Composition
 Glucose100g
 Yeast extract10g
 CaCO320g
 Agar (if needed)15g
 Distilled water1L
 Adjust pH to 6.8 
  20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.
 
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