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Medium Detail Information

Medium No.	1257
Medium	Medium for Strain SR45
Composition
KH2PO4 0.2 g   NH4Cl 0.25 g   NaCl 1 g   MgCl2·6H2O 0.4 g   KCl 0.5 g   CaCl2·2H2O 0.15 g   Na2SO4 4 g   Yeast extract 1 g   Resazurin 1 mg   Sodium acetate 2.73 g   Trace element mixture* 1 ml   Selenite-tungstate solution** 1 ml   Bicarbonate solution*** 30 ml   Sulfide solution**** 1.5 ml   Distilled water 970 ml   pH 6.8-7.0       Mix ingredients except for bicarbonate and sulfide solutions. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically and anaerobically add other ingredients using sterile syringes, and the N2/CO2 (80/20) mixed gas is enclosed with the vessels. Adjust pH to 6.8-7.0 using compact pH meter.                   *Trace element mixture       HCl (25%; 7.7 M) 12.5 ml       FeSO4·7H2O 2100 mg       H3BO3 30 mg       MnCl2·4H2O 100 mg       CoCl2·6H2O 190 mg       NiCl2·6H2O 24 mg       CuCl2·2H2O 2 mg       ZnSO4·7H2O 144 mg       Na2MoO4·2H2O 36 mg       Distilled water 987 ml     Sterilized by autoclaving.                   **Selenite-tungstate solution       NaOH 0.4 g       Na2SeO3·5H2O 6 mg       Na2WO4·2H2O 8 mg       Distilled water 1 L     Sterilized by autoclaving.                   ***Bicarbonate solution (1.0 M)       NaHCO3 8.4 g       Distilled water 100 ml     Saturated with CO2 gas. Tightly sealed in bottles filled with CO2 gas and then sterilized by autoclaving.                   ****Sulfide solution (1.0 M)       Na2S·9H2O 12 g       Distilled water 50 ml     Sterilized by filtration and stored under N2 gas.
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