カタログ検索結果 詳細情報

Medium Detail Information

Medium No.	1275
Medium	Modified Tc medium
Composition
NaCl 25 g   KCl 0.33 g   NH4Cl 0.25 g   MgCl2·6H2O 2.8 g   MgSO4·7H2O 3.4 g   FeSO4·7H2O 0.025 g   K2HPO4 0.3 g   NaBr 0.01 g   Trace elements solution* 10 ml   Vitamin solution** 10 ml   Bacto Yeast Extract (Difco) 3 g   Bacto Tryptone (Difco) 3 g   NaHCO3 2.5 g   Sulfur (powder) 3 g   Resazurin 1 mg   Na2S·9H2O 0.5 g   Distilled water 1 L     Mix ingredients except vitamin solution, sulfur and Na2S·9H2O, and autoclave under a N2/CO2 (80/20) atmosphere. Filter-sterilize vitamin solution. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each 3 successive days. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add vitamin, sulfur and Na2S·9H2O. Final pH is 6.8.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.8 mg       Na2WO4 0.8 mg       KAl(SO4)2·12H2O 4 mg       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH is 7.0.                   ** Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L
Comment