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Medium Detail Information

Medium No.	1323
Medium	GV-medium
Composition
K2HPO4 0.35 g   KH2PO4 0.23 g   NH4Cl 0.5 g   NaCl 2.25 g   FeSO4·7H2O 2 mg   Trace element solution* 1 ml   Selenite/tungstate solution** 1 ml   Yeast extract 1 g   Resazurin 0.5 mg   NaHCO3 3 g   MgSO4·7H2O 0.5 g   CaCl2·2H2O 0.25 g   D-Glucose 5 g   Vitamin solution*** 10 ml   L-Cysteine-HCl·H2O 0.3 g   Na2S·9H2O 0.3 g   Distilled water 1 L     Dissolve ingredients (except bicarbonate, magnesium sulfate, calcium chloride, glucose, vitamins, cysteine and sulfide), bring medium to the boil, and then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate, adjust pH to 7.0, dispense under same gas atmosphere in culture vessels and autoclave. After sterilization, add magnesium sulfate, calcium chloride, glucose, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2. Vitamins are prepared under 100% N2 gas and sterilized by filtration. Adjust pH of the completed medium to 7.0 - 7.2, if necessary.                   *Trace element solution.       HCl (25% or 7.7 M) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 6 mg       CoCl2·6H2O 190 mg       CuCl2·2H2O 2 mg       NiCl2·6H2O 24 mg       Na2MoO4·2H2O 36 mg       Distilled water 990 ml     First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000 ml.                   **Selenite-tungstate solution.       NaOH 0.5 g       Na2SeO3·5H2O 3 mg       Na2WO4·2H2O 4 mg       Distilled water 1 L                   ***Vitamin solution.       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl·2H2O 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       D-Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L
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