| |
Disodium succinate |
10 |
g |
| |
Na2HPO4 |
2.2 |
g |
| |
KH2PO4 |
0.8 |
g |
| |
NH4NO3 |
3 |
g |
| |
CaCl2·2H2O |
10 |
mg |
| |
MgCl2·6H2O |
10 |
mg |
| |
FeCl2·2H2O |
10 |
mg |
| |
Modified Hunter's vitamin-free mineral base* |
20 |
ml |
| |
Dimethyl sulfoxide** |
5 |
ml |
| |
Agarose (Electrophoresis grade, if needed) |
5 |
g |
| |
Distilled water |
975 |
ml |
| |
pH 7.2 |
|
| |
|
|
|
|
|
|
| |
|
*Modified Hunter's vitamin-free mineral base |
| |
|
|
Nitrilotriacetic acid (NTA) |
10 |
g |
| |
|
|
MgCl2·6H2O |
16 |
g |
| |
|
|
CaCl2·2H2O |
3.3 |
g |
| |
|
|
(NH4)6MoO7O24·4H2O |
9.25 |
mg |
| |
|
|
FeCl3·6H2O |
0.102 |
g |
| |
|
|
Mofified metals 44*** |
50 |
ml |
| |
|
|
Distilled water |
950 |
ml |
| |
|
Dissolve and neutralized nitrilotriacetic acid in 500 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8, and bring volume to 1 L with distilled water. |
| |
|
|
|
|
|
|
| |
|
**Dimethyl sulfoxide |
| |
| |
|
Sterilize separately by filtration. |
| |
|
|
|
|
|
|
| |
|
***Mofified metals 44 |
| |
|
|
Na-EDTA |
2.5 |
g |
| |
|
|
ZnCl2 |
5.17 |
g |
| |
|
|
FeCl2·6H2O |
4.85 |
g |
| |
|
|
MnCl2·4H2O |
2.02 |
g |
| |
|
|
CuCl2·2H2O |
0.267 |
g |
| |
|
|
Co(NO3)2·6H2O |
0.248 |
g |
| |
|
|
Na2B4O7·10H2O |
0.177 |
g |
| |
|
|
Distilled water |
1 |
L |
| |
| |
|
Dissolve the EDTA and add a few drops of 12M HCl to retard precipitation of the heavy metal ions. Store in the dark and cold (5℃). |