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Medium Detail Information

Medium No.	1336
Medium	Leptospirillum (HH) Medium
Composition
Solution A* 950 ml   Solution B** 50 ml   Trace element solution*** 1 ml   pH 1.8       Autoclave the solutions separately at 112°C for 30 min. Prior to use, mix solutions A and B, and add 1 ml of Trace elements solution. The final pH of the medium should be 1.8.                   *Solution A:       (NH4)2SO4 132 mg       MgCl2·6H2O 53 mg       KH2PO4 27 mg       CaCl2·2H2O 147 mg       Distilled water 950 ml   pH 1.8       Adjust pH to 1.8 with 10N H2SO4.                   **Solution B:       FeSO4·7H2O 20 g       H2SO4 Solution (0.25 N) 50 ml   pH 1.2       The pH of this solution should be 1.2.                   ***Trace element solution       MnCl2·2H2O 62 mg       ZnCl2 68 mg       CoCl2·6H2O 64 mg       H3BO3 31 mg       Na2MoO4 10 mg       CuCl2·2H2O 67 mg       Distilled water 1 L   pH 1.8       Adjust pH to 1.8 with 10N H2SO4.
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