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Medium No.	1353
Medium	M9 medium
Composition
10xM9 solution* 100 ml   MgSO4 solution (1 M)** 2.1 ml   CaCl2 solution (0.1 M)** 1 ml   Distilled water 900 ml     After autoclaving of distilled water, aseptically mix 10xM9, MgSO4 and CaCl2 solutions.                   *10xM9 solution       KH2PO4 30 g       Na2HPO4 60 g       NH4Cl 10 g       NaCl 5 g       Distilled water 1 L     Sterilize separately by autoclaving.                   **Dissolve in distilled water and sterilize by autoclaving.
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