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Medium Detail Information

Medium No.
1363 
Medium
PYG Medium 
Composition
 Bacto Tryptone (Difco)5g
 Bacto Peptone (Difco)5g
 Bacto Yeast Extract (Difco)10g
 Bact Beef Extract (Difco)5g
 Glucose5g
 K2HPO42g
 Cysteine-HCl·H2O0.5g
 Tween 801ml
 Resazurin1mg
 Salt solution*40ml
 Hemin solution**10ml
 Menadione solution***10ml
 Distilled water940ml
 pH 7.2 
  Dissolve ingredients except cysteine-HCl, hemin and menadione solutions, and adjust the pH to 7.2. Dispense the medium into suitable culture vessels under a stream of N2 gas and seal with butyl rubber stoppers. After autoclaving (121°C, 15 min), aseptically and anaerobically add the other ingredients.
       
  *Salt solution
   CaCl2·2H2O0.25g
   MgSO4·7H2O0.5g
   K2HPO41g
   KH2PO41g
   NaHCO310g
   NaCl2g
   Distilled water1L
       
  **Hemin solution
  Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.
       
  ***Menadione solution
  Dissolve 5 mg menadione first in 1 ml of ethanol. Then adjust volume to 100 ml.
 
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