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Medium Detail Information

Medium No.	1378
Medium	Modified synthetic Crenarchaeota medium for strain SCM1
Composition
NaCl 26 g   MgSO4·7H2O 5 g   MgCl2·6H2O 5 g   CaCl2·2H2O 1.5 g   KBr 0.1 g   HEPES buffer* 10 ml   1 M NaHCO3 buffer** 5 ml   KH2PO4 solution*** 5 ml   FeNaEDTA solution**** 5 ml   Trace element solution***** 1 ml   NH4Cl solution****** 2.5 ml   Sodium alpha-Ketoglutamate solution******* 5 ml   Distilled water 1 L       Mix ingredients except for HEPES buffer, NaHCO3 buffer, KH2PO4 solution, FeNaEDTA solution, trace element solution, NH4Cl solution and alpha-ketoglutamate solution, and autoclave at 121°C for 20 min. HEPES buffer, and KH2PO4, FeNaEDTA, trace element, and NH4Cl solutions are sterilized separately by autoclaving and added just before inoculation. NaHCO3 buffer and alpha-ketoglutamate sollution are also added just before inoculation after filter-sterile. Final pH should be 7.4-7.6.                   *HEPES buffer       NaOH 24 g       HEPES 238.4 g       Distilled water 1 L       Dissolve NaOH, and then add HEPES slowly. Adjust pH to 7.6 with NaOH solution. Sterilize by autoclaving.                   **1 M NaHCO3 buffer       Dissolve NaHCO3, and sterilize by filter.                   ***KH2PO4 solution       Dissolve KH2PO4 (0.4 g/L=2.93 mM), and sterilize by autoclaving.                   ****FeNaEDTA solution       Dissolve FeNaEDTA (578 mg/L=1.5 mM), and sterilized by autoclaving. Store at 4°C.                   *****Trace element solution       HCl (35%) 9 ml       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.19 g       ZnSO4·7H2O 0.144 g       CuCl2·2H2O 0.002 g       H3BO3 0.03 g       Na2MoO4·2H2O 0.036 g       NiCl2·6H2O 0.024 g       Distilled water 1 L       Add HCl, dissolve trace elements, and sterilize by autoclaving. Store at 4°C.                   ******NH4Cl solution       Dissolve NH4Cl (0.4 M), and sterilize by autoclaving.                   *******Sodium alpha-ketoglutamate solution       Dissolve alpha-ketoglutamate (20 mM), and sterilize by filter. Store at 4°C.
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