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Medium Detail Information

Medium No.	1381
Medium	PPM
Composition
MES 2 g   MgSO4·7H2O 1 g   NH4H2PO4 0.053 g   Xylan from Beechwood 0.05 g   Pectin 0.05 g   Bacto Yeast Extract (Difco) 0.05 g   Bacto Casamino Acids (Difco) 0.05 g   Trace element solution (SL-10)* 2 ml   Se/W solution** 2 ml   Cycloheximide 0.1 g   Gelrite (if needed) 8 g   Distilled water 1 L   pH 5.0         For solid medium, equal volumes of separately autoclaved (115°C for 10 min) 1.6% gelrite and double-strength liquid medium are mixed.                   *Trace element solution (SL-10)       HCl (25%) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 0.07 g       MnCl2·4H2O 0.1 g       B(OH)3 0.006 g       CoCl2·6H2O 0.19 g       CuCl2·2H2O 0.002 g       NiCl2·6H2O 0.024 g       Na2MoO4·2H2O 0.036 g       Distilled water 1 L                   **Se/W solution       NaOH 0.4 g       Na2SeO3 0.002 g       Na2WO4 0.004 g       Distilled water 1 L
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