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Medium Detail Information

Medium No.	1392
Medium	Modified Methanoculleus Medium
Composition
NH4Cl 0.5 g   MgSO4·7H2O 4.0 g   CaCl2·2H2O 0.14 g   K2HPO4 0.14 g   KCl 0.1 g   NaCl 6 g   Fe(NH4)2(SO4)2·6H2O 2 mg   (NH4)2Ni(SO4)2·6H2O 2 mg   Vitamin solution* 10 ml   Trace elements solution** 10 ml   Se/W/AI solution*** 2 ml   Glucose 5 g   Resazurin 1 mg   NaHCO3 2.5 g   Cysteine-HCl 0.5 g   Na2S·9H2O 0.5 g   Distilled water 1 L       Mix ingredients except for vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under N2/CO2 (80/20) condition. Separately autoclave cysteine-HCl and Na2S·9H2O as a 5% solution under a N2 atmosphere. Aseptically and anaerobically add cysteine-HCl and Na2S·9H2O solutions, and filter-sterile vitamin solution.                   *Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L                   **Trace element solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 24 mg       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 25 mg       H3BO3 10 mg       Na2MoO4·2H2O 24 mg       NaCl 1 g       NiCl2·6H2O 0.12 g       Distilled water 1 L       First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, then add minerals. Final pH 7.0.                   ***Se/W/AI solution       Na2SeO3 4 mg       Na2WO4 4 mg       KAl(SO4)2·12H2O 20 mg       Distilled water 1 L
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