|
Na2SO4 |
0.0142 |
g |
|
NH4Cl |
0.0403 |
g |
|
MgCl2·6H2O |
0.0203 |
g |
|
K2HPO4 |
0.0401 |
g |
|
CaCl2·2H2O |
0.29 |
g |
|
CaCO3 |
2 |
g |
|
CAPS |
3.32 |
g |
|
Sodium acetate |
0.33 |
g |
|
Vitamin solution* |
10 |
ml |
|
Trace element solution** |
10 |
ml |
|
Distilled water |
1 |
L |
|
pH 11 |
|
|
|
|
Mix ingredients except for vitamin solution, adjust pH to 11 with NaOH, and autoclave under a N2 atmosphere. Add the filter-sterile vitamin solution, and exchange N2 to a gas mixture of N2/H2/O2 (59/35/6) in the headspace. |
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|
|
|
|
|
|
|
|
*Vitamin solution |
|
|
|
Biotin |
2 |
mg |
|
|
|
Folic acid |
2 |
mg |
|
|
|
Pyridoxine-HCl |
10 |
mg |
|
|
|
Thiamine-HCl |
5 |
mg |
|
|
|
Riboflavin |
5 |
mg |
|
|
|
Nicotinic acid |
5 |
mg |
|
|
|
Ca-pantothenate |
5 |
mg |
|
|
|
p-Aminobenzoic acid |
1 |
mg |
|
|
|
Vitamin B12 |
0.01 |
mg |
|
|
|
Distilled water |
1 |
L |
|
|
|
|
|
|
|
|
|
**Trace elements solution |
|
|
|
Nitrilotriacetic acid (NTA) |
12.8 |
g |
|
|
|
FeCl3·6H2O |
1.35 |
g |
|
|
|
MnCl2·4H2O |
0.1 |
g |
|
|
|
CoCl2·6H2O |
0.024 |
g |
|
|
|
CaCl2·2H2O |
0.1 |
g |
|
|
|
ZnCl2 |
0.1 |
g |
|
|
|
CuCl2·2H2O |
0.025 |
g |
|
|
|
H3BO3 |
0.01 |
g |
|
|
|
Na2MoO4·2H2O |
0.024 |
g |
|
|
|
NaCl |
1 |
g |
|
|
|
NiCl2·6H2O |
0.12 |
g |
|
|
|
Na2SeO4 |
0.004 |
g |
|
|
|
Na2WO4 |
0.004 |
g |
|
|
|
KAl(SO4)2·12H2O |
0.02 |
g |
|
|
|
Distilled water |
1 |
L |
|
|
|
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0. |
|