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Medium No.	1411
Medium	VY/2-S75-15
Composition
Yeast cell paste* (wet weight) 5.0 g   Yeast extract 0.1 g   NaCl 15.0 g   Cyanocobalamin** 0.5 mg   SWS solution*** 750 ml   Distilled water 250 ml   Agar 15.0 g   pH 7.2-7.4                     *Wash dry yeast with distilled water and discard supernatant by centrifugation.                   **Add 1 ml of filter sterilized cyanocobalamin solution (0.5 mg/ml) after autoclaving and cooling.                   ***SWS (Sea Water Salts) solution       Ferric citrate 0.01 g       MgSO4·7H2O 8.00 g       CaCl2·2H2O 1.00 g       KCl 0.50 g       NaHCO3 0.16 g       H3BO3 0.02 g       KBr 0.08 g       SrCl2·6H2O 0.03 g       di-Na-s-glycerophosphate 0.01 g       Trace elements solution**** 1.00 ml       Distilled water 1.00 L                   ****Trace element solution       MnCl2·4H2O 100 mg       CoCl2 20 mg       CuSO4 10 mg       Na2MoO4·2H2O 10 mg       ZnCl2 20 mg       LiCl 5 mg       SnCl2·2H2O 5 mg       H3BO3 10 mg       KBr 20 mg       KI 20 mg       Fe(III)-EDTA 8 g       Distilled water 1 L
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