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Medium Detail Information

Medium No.	1412
Medium
Composition
Trypticase peptone 10 g   Yeast extract 5 g   Glucose 10 g   K2HPO4 0.45 g   KH2PO4 0.45 g   (NH4)2SO4 0.9 g   NaCl 0.9 g   MgSO4·7H2O 0.09 g   CaCl2·2H2O 0.09 g   Resazurin 1 mg   Na2CO3 0.2 g   Cysteine-HCl·H2O 0.3 g   Distilled water 1 L     Mix ingredients except cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of N2 gas and seal with butyl rubber stoppers. Separately autoclave cysteine-HCl as 5% solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine-HCl solution. pH after autoclaving is 6.7-7.0.
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