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Medium Detail Information

Medium No.	1431
Medium	HSO medium
Composition
K2HPO4 0.14 g   NH4Cl 0.25 g   MgCl2·6H2O 4.2 g   MgSO4·7H2O 3.4 g   NaCl 25 g   KCl 0.5 g   CaCl2·2H2O 0.7 g   NaHCO3 1 g   Bacto Yeast Extract (Difco) 1 g   Sodium acetate 0.5 g   Trace element solution* 10 ml   Resazurin 1 mg   Sulfur (powder) 10 g   Na2S·9H2O 0.5 g   Distilled water 1 L     Mix ingredients except for sulfur and Na2S·9H2O, and autoclave under a H2/CO2 atmosphere (80/20). Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Separately autoclave and neutralize Na2S·9H2O as 5% solution under a N2 atmosphere. Prior to inoculation, add sulfur and Na2S·9H2O. Pressurize the inoculated vessels to 150 kPa with H2/CO2 (80/20).                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.004 g       Na2WO4 0.004 g       KAl(SO4)2·12H2O 0.02 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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