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Medium Detail Information

Medium No.	1441
Medium	Petrothermobacter Medium
Composition
Sodium pyruvate 2.2 g   NH4Cl 0.54 g   KH2PO4 0.14 g   MgCl2·6H2O 3 g   CaCl2·2H2O 0.15 g   NaCl 25 g   NaHCO3 2.5 g   Trace element solution* 2 ml   Vitamin solution** 2 ml   Cysteine-HCl 0.3 g   Na2S·9H2O 0.3 g   Distilled water 1 L     Mix ingredients except for sodium pyruvate, vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclaved under a N2/CO2 atmosphere (80/20). Concentrated cysteine-HCl and Na2S·9H2O solutions are sterilized separately under a N2 atmosphere in tightly closed vessels. Prior to inoculation, add filter-sterile vitamin and sodium pyruvate solutions, cysteine-HCl, and Na2S·9H2O. Final pH is 7.                   *Trace element solution       HCl (35%) 7 ml       FeCl3·6H2O 1500 mg       H3BO3 6 mg       MnCl2·4H2O 100 mg       CoCl2·6H2O 190 mg       NiCl2·6H2O 24 mg       CuCl2·2H2O 2 mg       ZnCl2 70 mg       Na2MoO4·2H2O 36 mg       Na2SeO4 4 mg       Na2WO4 4 mg       KAl(SO4)2·12H2O 20 mg       Distilled water 1 L                   **Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 5 mg       Distilled water 1 L
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