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Medium Detail Information

Medium No.	1458
Medium	MWMM medium
Composition
NH4Cl 0.2 g   MgSO4·7H2O 0.04 g   CaCl2·2H2O 0.02 g   K2HPO4 0.01 g   Trace element solution* 1 ml   Se/W solution** 1 ml   Vitamin solution*** 2 ml   NaHCO3 0.42 g   FeSO4·7H2O 0.278 g   Distilled water 1 L     Mix ingredients except for vitamin solution, NaHCO3 and FeSO4·7H2O, and autoclave under a N2/CO2 atmosphere (80/20). Just prior to inoculation, add the filter-sterile vitamin, NaHCO3 and FeSO4·7H2O solutions. In addition, add filter-sterile O2 as the final concentration 0.5% in the headspace.                   *Trace element solution       HCl (35%) 7 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 30 mg       CoCl2·6H2O 190 mg       CuCl2·2H2O 2 mg       NiCl2·6H2O 24 mg       Na2MoO4·2H2O 36 mg       Distilled water 1 L                   **Se/W solution       NaOH 0.5 g       Na2SeO3·5H2O 3 mg       Na2WO4·2H2O 4 mg       distilled water 1 L                   ***Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 0.01 mg       Distilled water 1 L
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