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Medium Detail Information

Medium No.
 1507
Medium
 Modified M30 Medium
Composition
Solution 1* 20 ml
Solution 2*** 50 ml
0.1 M Tris/HCl (pH7.5) 50 ml
MgCl2⋅6H2O 0.5 g
Glucose 1 g
Agar 20 g
Artificial seawater 700 ml
Distilled water 180 ml

Mix ingregients except Solution 2, adjust pH to 7.5 and autoclave at 121°C for 20 min. After autoclaving, add filter-sterilized Solutin 2.

*Solution 1

Nitrilotriacetic acid (NTA) 10 g
MgSO4⋅7H2O 29.7 g
CaCl2⋅7H2O 3.3 g
(NH4)6MoO7O24⋅4H2O 9.3 mg
FeSO4⋅7H2O 99 mg
Trace element solution** 50 ml
Distilled water 950 ml
First dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH, then add other salts. Adjust the final pH to 6.8 with NaOH or H2SO4.

**Trace element solution

Nitrilotriacetic acid (NTA) 12.8 g
FeCl2⋅6H2O 1.35 g
MnCl2⋅4H2O 0.1 g
CoCl2⋅6H2O 0.024 g
CaCl2⋅2H2O 0.1 g
ZnCl2 0.1 g
ZnSO4⋅7H2O 1.1 g
CuCl2⋅4H2O 0.025 g
H3BO3 0.01 g
Na2MoO4⋅2H2O 0.024 g
NaCl 1 g
NiCl2⋅6H2O 0.12 g
Na2B4O7⋅10H2O 0.018 g
Distilled water 1 L
 

First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, then add other salts.Final pH 7.0 with NaOH.

***Solution 2

N-acetylglucosamine 2 g
Na2HPO4⋅2H2O 0.01 g
Vitamin solution**** 10 ml
Distilled water 40 ml






Adjust pH to 7.5 and filter-sterilize.

****Vitamin solution

Biotin 2 mg
Folic acid 2 mg
Pyridoxine-HCl (Vitamin B6) 10 mg
Thiamine-HCl⋅H2O (Vitamin B1) 5 mg
Riboflavin (Vitamin B2) 5 mg
Nicotinic acid 5 mg
D-Ca-pantothenate 5 mg
Vitamin B12 0.1 mg
p-Aminobenzoic acid 5 mg
Lipoic acid 5 mg
Distilled water 1 L















Adjust pH to 7.0 with NaOH.
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