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Medium Detail Information

Medium No.
1514
Medium
Desulfovibrio Medium
Composition
NH4Cl 1 g
Na2SO4 2 g
Na2S2O3·5H2O 1 g
MgSO4·7H2O 1 g
CaCl2·2H2O 0.1 g
KH2PO4 0.5 g
Trace element solution SL-10* 1 mL
Se/W solution** 1 mL
Bacto Yeast Extract (Difco) 1 g
Sodium lactate 2.5 g
Vitamin solution*** 10 mL
Resazurin 0.5 mg
NaHCO3 2 g
Na2S·9H2O 0.1 g
Distilled water 1 L

Mix ingredients except for sodium lactate, vitamin solution, NaHCO3 and Na2S·9H2O. Dispense the medium into suitable culture vessels and autoclave under a N2 atmosphere. Separately autoclave concentrated sulfide solution under a N2 atmosphere. Sterilize lactate, vitamin and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add lactate, vitamin, NaHCO3 and sulfide solutions. Final pH is 7.2-7.5.

*Trace element solution SL-10

HCl (35%) 7 mL
FeCl3·6H2O 1.5 g
ZnCl2 70 mg
MnCl2·4H2O 100 mg
H3BO3 6 mg
CoCl2·6H2O 190 mg
CuCl2·2H2O 2 mg
NiCl2·2H2O 24 mg
Na2MoO4·2H2O 36 mg
Distilled water 1 L

First dissolve FeCl3 in concentrated HCl, dilute in water, and then add the other minerals.

**Se/W solution

Na2SeO4 4 mg
Na2WO4 4 mg
Distilled water 1 L





***Vitamin solution

Biotin 2 mg
Folic acid 2 mg
Pyridoxine-HCl (Vitamin B6) 10 mg
Thiamine-HCl·H2O (Vitamin B1) 5 mg
Riboflavin (Vitamin B2) 5 mg
Nicotinic acid 5 mg
D-Ca-Pantothenate 5 mg
Cyanocobalamin (Vitamin B12) 0.1 mg
p-Aminobenzoic acid 5 mg
Lipoic acid 5 mg
Distilled water 1 L















Adjust pH to 7.0 with NaOH.

 

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