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Medium Detail Information

Medium No.
1550
Medium
Denitrovibrio Medium
Composition
Na2SO4 0.02 g
KH2PO4 1 g
NH4Cl 0.25 g
NaCl 20 g
MgCl2·6H2O 3 g
KCl 0.5 g
CaCl2·2H2O 0.15 g
Sodium acetate 1.64 g
Disodium fumarate 6.4 g
Trace element solution SL-10* 1 mL
Vitamin solution** 10 mL
Resazurin 0.5 mg
Na2CO3 1 g
Na2S·9H2O 0.5 g
Distilled water 1 L

Mix ingredients except fumarate, vitamin, carbonate and sulfide, and autoclave under a N2/CO2 atmosphere (80/20). Concentrated fumarate and sulfide solutions are sterilized under a N2 atmosphere in tightly closed vesseles. Prior to inoculation, add filter-sterile vitamin and carbonate solutions, fumarate and sulfide. Final pH is 6.8-7.2.

*Trace element solution SL-10

HCl (35%) 7 mL
FeCl3·6H2O 1.5 g
ZnCl2 70 mg
MnCl2·4H2O 100 mg
H3BO3 6 mg
CoCl2·6H2O 190 mg
CuCl2·2H2O 2 mg
NiCl2·2H2O 24 mg
Na2MoO4·2H2O 36 mg
Na2SeO4 4 mg
Na2WO4 4 mg
Distilled water 1 L

First dissolve FeCl3 in concentrated HCl, dilute in water, and then add the other minerals.

**Vitamin solution

Biotin 2 mg
Folic acid 2 mg
Pyridoxine-HCl (Vitamin B6) 10 mg
Thiamine-HCl·H2O (Vitamin B1) 5 mg
Riboflavin (Vitamin B2) 5 mg
Nicotinic acid 5 mg
D-Ca-Pantothenate 5 mg
Cyanocobalamin (Vitamin B12) 0.1 mg
p-Aminobenzoic acid 5 mg
Lipoic acid 5 mg
Distilled water 1 L
  Adjust pH to 7.0 with NaOH.  


















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