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Medium Detail Information

Medium No.	1562
Medium	Cedears Standard Medium 3
Composition
Na2SO4 0.0071 g   NH4Cl 0.02 g   MgCl2·6H2O 0.01 g   K2HPO4 0.02 g   CaCl2·2H2O 0.147 g   Ca3(PO4)2 0.372 g   CAPS 3.32 g   Sodium acetate 0.164 g   Vitamin solution* 10 ml   Trace element solution** 10 ml   Distilled water 1 L   pH 11         Mix ingredients except for vitamin solution, adjust pH to 11 with NaOH, and autoclave under a N2 atmosphere. Add the filter-sterile vitamin solution, and exchange N2 to a gas mixture of N2/H2/O2 (59/35/6) in the headspace.                   *Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 0.01 mg       Distilled water 1 L                   **Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.004 g       Na2WO4 0.004 g       KAl(SO4)2·12H2O 0.02 g       Distilled water 1 L       First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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