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Medium No.	233
Medium
Composition
Methanol* 10 ml   (NH4)2HPO4 3 g   NaCl 1 g   MgSO4·7H2O 0.2 g   FeSO4·7H2O 10 mg   MnSO4·nH2O 5 mg   Vitamin mixture** 5 ml   Tap water 1 L   Agar (if needed)*** 20 g   pH 7.0                     *Sterilize by filtration and add to other autoclaved ingredients after cooling.                   **Vitamin mixture       Biotin 0.2 mg       Ca-pantothenate 40 mg       Folic acid 0.2 mg       myo-Inositol 200 mg       Niacin 40 mg       p-Aminobenzoic acid 20 mg       Pyridoxine-HCl 40 mg       Riboflavin 40 mg       Thiamin-HCl 20 mg       Choline chloride 100 mg       Cyanocobalamin 2 μg       Distilled water 1 L                   ***Sterilize separately.
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