カタログ検索結果 詳細情報

Medium Detail Information

Medium No.	263
Medium
Composition
NaCl 100 g   MgCl2·6H2O 5 g   MgSO4·7H2O 9.5 g   KCl 5 g   CaCl2·2H2O 0.2 g   (NH4)2SO4 0.1 g   KNO3 0.1 g   Bacto Casamino Acids (Difco) 5 g   Yeast extract 1 g   HMSS solution* 20 ml   PS solution** 20 ml   Vitamin mixture*** 10 ml   Distilled water 960 ml   Agar (if needed) 15 g   pH 8.0                     *HMSS solution       Nitrilotriacetic acid 10 g       MgSO4·7H2O 29.7 g       CaCl2·2H2O 3.3 g       Na2MoO4·2H2O 12.7 mg       FeSO4·7H2O 99 mg       Metal 44 solution**** 50 ml       Distilled water 1 L     Neutralize the nitrilotriacetic acid with KOH. Dissolve the remaining ingredients and adjust the pH to 7.2 with KOH.                   **PS solution       K2HPO4 2.5 g       KH2PO4 2.5 g       Distilled water 1 L     Sterilize separately, and add after autoclaving.                   ***Vitamin mixture       Biotin 0.2 mg       Ca-pantothenate 40 mg       Folic acid 0.2 mg       myo-Inositol 200 mg       Niacin 40 mg       p-Aminobenzoic acid 20 mg       Pyridoxine-HCl 40 mg       Riboflavin 40 mg       Thiamin-HCl 20 mg       Choline chloride 100 mg       Cyanocobalamin 2 μg       Distilled water 1 L                   ****Metal 44 solution       2Na-EDTA 2.5 g       ZnSO4·7H2O 10.95 g       FeSO4·7H2O 5 g       MnSO4·nH2O 1.54 g       CuSO4·5H2O 0.392 g       CoCl2·6H2O 0.203 g       Na2B4O7·10H2O 0.177 g       Distilled water 1 L
Comment