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Medium Detail Information

Medium No.
 287 
Medium
  
Composition
 (NH4)2SO41.3g
 CaCl2·2H2O0.08g
 MgSO4·7H2O0.25g
 KH2PO40.28g
 Na2SiO3·9H2O0.1g
 Na2MoO4·2H2O0.03g
 FeSO4·7H2O0.02g
 Yeast extract*1g
 Sulfur (powder)**10g
 Distilled water1L
 Adjust pH to 2.5 with 6 N H2SO4 
       
  *Yeast extract is autoclaved separately as 10% solution.
       
  **Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days and added after inoculation.
 
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