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Medium Detail Information

Medium No.	377
Medium	Oceanithermus medium
Composition
KH2PO4 0.75 g   K2HPO4 0.78 g   MgCl2·6H2O 0.36 g   CaCl2·2H2O 0.1 g   NH4Cl 0.54 g   NaHCO3 5 g   Trace element solution* 10 ml   Vitamin solution** 10 ml   Yeast extract 2 g   NaNO3 1.7 g   NaCl 20 g   Distilled water 1 L   pH unadjusted       Mix ingredients, except NaHCO3 and vitamin solution. Dispense the medium into suitable culture vessels under a stream of N2 and seal with butyl rubber stoppers. After autoclaving, add filter-sterile NaHCO3 and vitamin solutions.                   *Trace elements solution       2Na-EDTA 0.64 g       MgSO4·7H2O 6.2 g       MnSO4·4H2O 0.55 g       NaCl 1 g       FeSO4·7H2O 0.1 g       CoCl2·6H2O 0.17 g       CaCl2·2H2O 0.13 g       ZnSO4·7H2O 0.18 g       CuSO4 0.05 g       KAl(SO4)2·12H2O 0.018 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.011 g       NiCl2·6H2O 0.025 g     Dissolve the 2Na-EDTA in 500 ml of distilled water, and after adding the salts, make up to 1 L.                   **Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 0.01 mg       Distilled water 1 L
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