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Medium Detail Information

Medium No.	383
Medium	2.5% NaCl GYPFK Agar/Broth
Composition
Solution A       Glucose 10 g       Hipolypepton* 5 g       Yeast extract 5 g       Extract Bonito* 5 g       NaCl 25 g       K2HPO4 10 g       Salt solution** 5 ml       Agar (if needed) 15 g       Distilled water 900 ml   pH unadjusted       Solution B       Na2CO3 3 g       Distilled water 100 ml   pH unadjusted       Solutions A and B are separately sterilized by autoclaving and mix aseptically.     Final pH around 8.5.                   *Wako Pure Chemical Ind., Ltd., Osaka, Japan.                   **Salt solution       MgSO4·7H2O 40 g       MnSO4·4H2O 2 g       FeSO4·7H2O 2 g       Distilled water 1 L
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