| | Bacto Yeast Extract (Difco) | 1 | g | | | Trypticase Peptone (BBL) | 1 | g | | | Na2S2O3·5H2O | 1 | g | | | Sea water | 1 | L | | | pH 7.0 - 7.2 | | | | | | Use filtrated natural seawater or artificial seawater (e.g. Aquamarine, Yashima Co.). Separately sterilize a solution of Na2S2O3·5H2O by filtration, and aseptically add to the medium. |
|