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Medium Detail Information

Medium No.	851
Medium	Thermotoga maritima Medium
Composition
Soluble starch 5 g   KH2PO4 0.5 g   Trace minerals* 10 ml   NaCl 20 g   Bacto Yeast Extract (Difco) 0.5 g   Resazurin 1 mg   Na2S·9H2O** 0.5 g   Artificial sea water*** 250 ml   Distilled water 750 ml     Mix ingredients except Na2S·9H2O, adjust pH to 6.5 with NaOH, and autoclave under a N2 atmosphere. Separately autoclave and neutralize Na2S·9H2O as 5% solution under a N2 atmosphere. Anaerobically add Na2S·9H2O solution.                   *Trace minerals       Nitrilotriacetic acid 1.5 g       MgSO4·7H2O 3 g       MnSO4·2H2O 0.5 g       NaCl 1 g       FeSO4·7H2O 0.1 g       CoSO4·7H2O 0.18 g       CaCl2·2H2O 0.1 g       ZnSO4·7H2O 0.18 g       CuSO4·5H2O 0.01 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.01 g       NiCl2·6H2O 0.025 g       Na2SeO3·5H2O 0.8 mg       Na2WO4 0.8 mg       KAl(SO4)2·12H2O 4 mg       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).                   **The sulfide is prepared in a vessel sealed with a butyl rubber stopper. Autoclave under a N2 atmosphere. After cooling, the pH is adjusted to about 7.3 by adding of sterile 4-0.2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow color indicate the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.                   ***Artificial sea water       NaCl 27.7 g       MgSO4·7H2O 7 g       MgCl2·6H2O 5.5 g       CaCl2·2H2O 2.25 g       KCl 0.65 g       NaBr 0.1 g       H3BO3 30 mg       SrCl2·6H2O 15 mg       Citric acid 10 mg       KI 0.05 mg       Distilled water 1 L
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