| Marine medium/synthetic seawater mix solution* | 125 | ml |
| Trace elements** | 10 | ml |
| NH4Cl | 0.25 | g |
| NaHCO3 | 2.2 | g |
| KH2PO4 | 0.07 | g |
| Fe(NH4)2(SO4)2·6H2O | 2 | mg |
| (NH4)2Ni(SO4)2·6H2O | 2 | mg |
| Bacto Yeast Extract (Difco) | 0.5 | g |
| Na2S2O3·5H2O | 1 | g |
| Distilled water | 865 | ml |
|
| | After flushing with N2/CO2, adjust pH to 7.0 with 1 N H2SO4. Sterilize by filtration. Dispense the medium into vessels under a N2/CO2/O2 (79/20/1) atmosphere. Pressurize the inoculated vessels to 200 kPa with N2/CO2/O2. |
| | | | | | |
| | *Marine medium/synthetic seawater mix solution |
| | | NaCl | 47 | g |
| | | MgCl2·6H2O | 18 | g |
| | | MgSO4·7H2O | 7 | g |
| | | CaCl2·2H2O | 3 | g |
| | | Na2SO4 | 3.13 | g |
| | | Na2CO3 | 0.1 | g |
| | | NaBr | 0.2 | g |
| | | SrCl2·6H2O | 72 | mg |
| | | H3BO3 | 52 | mg |
| | | Na2HPO4 | 8.1 | mg |
| | | NaF | 2.4 | mg |
| | | KI | 0.05 | mg |
| | | Sodium silicate | 0.4 | g |
| | | Distilled water | 1 | L |
| | | | | | |
| | **Trace elements |
| | | Nitrilotriacetic acid | 1.5 | g |
| | | MgSO4·7H2O | 3 | g |
| | | MnSO4·2H2O | 0.5 | g |
| | | NaCl | 1 | g |
| | | FeSO4·7H2O | 0.1 | g |
| | | CoSO4·7H2O | 0.18 | g |
| | | CaCl2·2H2O | 0.1 | g |
| | | ZnSO4·7H2O | 0.18 | g |
| | | CuSO4·5H2O | 0.01 | g |
| | | H3BO3 | 0.01 | g |
| | | Na2MoO4·2H2O | 0.01 | g |
| | | NiCl2·6H2O | 0.025 | g |
| | | Na2SeO3·5H2O | 0.4 | mg |
| | | Na2WO4 | 0.4 | mg |
| | | KAl(SO4)2·12H2O | 2 | mg |
| | | Distilled water | 1 | L |
| | First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). |