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Medium Detail Information

Medium No.	900
Medium	Thermanaeromonas Medium
Composition
K2HPO4 0.78 g   KH2PO4 0.75 g   3Na-EDTA 0.04 g   FeSO4·7H2O 0.01 g   MgSO4·7H2O 0.25 g   CaCl2·2H2O 0.03 g   NaCl 0.2 g   NH4Cl 0.5 g   NaHCO3 5 g   Na2S2O3 0.8 g   Glucose 0.5 g   Bacto Yeast Extract (Difco) 0.5 g   Vitamin solution* 10 ml   Trace element solution** 10 ml   Cysteine-HCl 0.5 g   Distilled water 1 L     Mix ingredients except vitamin solution and cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20), seal with butyl rubber stoppers and autoclave. Separately autoclave 5% cysteine-HCl solution under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, aseptically add vitamin and cysteine-HCl solutions.                   *Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L                   **Trace element solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO3·5H2O 0.026 g       Distilled water 1 L
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