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Medium Detail Information

Medium No.	993
Medium	MMJS medium
Composition
NaCl 30 g   K2HPO4 0.14 g   CaCl2·2H2O 0.14 g   NH4Cl 0.125 g   NaNO3 0.125 g   MgSO4·7H2O 3.4 g   MgCl2·6H2O 4.2 g   KCl 0.33 g   Fe(NH4)2(SO4)2·6H2O 0.01 g   Bacto Yeast Extract (Difco) 1 g   Trace elements solution* 10 ml   Vitamin solution** 2 ml   NaHCO3 0.1 g   Sulfur (powder) 30 g   Resazurin 1 mg   Na2S·9H2O 0.5 g   Distilled water 1 L     Mix ingredients except for vitamin solution, sulfur, NaHCO3, and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of H2/CO2 (80/20), seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Separately autoclave Na2S·9H2O (5% solution) under N2 atmosphere. Sterilize vitamin and NaHCO3 solutions by filtration. Sulfur is sterilized separately by autoclaving for 60 min at 105°C on each of 3 successive days. Prior to inoculation, aseptically and anaerobically add vitamin, sulfur, NaHCO3, and Na2S·9H2O, and pressurize the inoculated vessels to 150 kPa with H2/CO2 (80/20). Final pH is 5.5-6.0.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.8 mg       Na2WO4 0.8 mg       KAl(SO4)2·12H2O 4 mg       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH is 7.0.                   **Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 0.01 mg       Distilled water 1 L
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