| | Solution A* | 500 | ml | | | Solution B** | 500 | ml | | | | | | | | | | | | *Solution A | | | | | Yeast extract | 2 | g | | | | | Distilled water | 500 | ml | | | Adjust pH to 4.5 with H2SO4 | | | | | | | | | | | | | **Solution B | | | | | Soluble starch | 2 | g | | | | | Glucose | 1 | g | | | | | Agar (if needed) | 15 | g | | | | | Distilled water | 500 | ml | | | | | Solutions A and B are separately sterilized by autoclaving and mixed. | | | | | Check the final pH around 4.5. |
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