NITE Biological Resource Center / Microbial Genomic DNAs

Microbial Genomic DNAs

NBRC is distributing to both academia and industries the microbial genomic DNA extracted from NBRC cultures. Ordering procedure is shown below of this page.

Genomic DNAs of the following microorganisms are available;
NBRC Number	Organism	Strain	Remarks
Bacteria
13948G	Clostridium acetobutylicum	NBRC 13948 = 2291 = ATCC 824	Type strain Sequenced genome [DDBJ] The average length is over 10kbp.
12016G	Clostridium kluyveri	NBRC 12016 = K-1 = DSM 555	Type strain Sequenced genome [DDBJ]
103400G	Clostridium thermocellum	NBRC 103400 = VPI 7372 = ATCC 27405	Type strain Sequenced genome [DDBJ]
100395G	Corynebacterium efficiens	NBRC 100395 = YS-314	Type strain Sequenced genome [DOGAN] [DDBJ]
12168G	Corynebacterium glutamicum	NBRC 12168 = 534	Type strain Sequenced genome [DDBJ]
12713G	Escherichia coli	NBRC 12713 = W3110	Sequenced genome [DDBJ]
16485G	Hydrogenobacter subterraneus	NBRC 16485 = HGP1	Type strain
14940G NEW!!	Klebsiella pneumoniae subsp. pneumoniae	NBRC 14940 = KM 2924 = NCTC 9633	Type strain
14854G	Mycoplasma fermentans	NBRC 14854 = PG18	Type strain The minimum quantity is 500ng. 16S-23S ITS region was checked by PCR method.⁽¹⁾
14477G	Mycoplasma orale	NBRC 14477 = CH19299	Type strain The minimum quantity is 500ng. 16S-23S ITS region was checked by PCR method.⁽¹⁾
14401G	Mycoplasma pneumoniae	NBRC 14401 = FH	Type strain The minimum quantity is 500ng. 16S-23S ITS region was checked by PCR method.⁽¹⁾ The average length is over 10kbp. Reference: Genome sequence of M. pneumoniae M129 [DDBJ]
13245G NEW!!	Salmonella enterica subsp. enterica	NBRC 13245 = LT 2 = TM 2	Type strain Sequenced genome [DDBJ]
Archaea
100138G	Aeropyrum pernix	NBRC 100138 = K1	Type strain Sequenced genome [DOGAN] [DDBJ]
100126G	Archaeoglobus fulgidus	NBRC 100126 = VC-16 = DSM 4304	Type strain Sequenced genome [DDBJ] The average length is over 10kbp.
100127G NEW!!	Archaeoglobus profundus	NBRC 100127 = AV18 = DSM 5631	Type strain The average length is under 10kbp.
100440G	Methanocaldococcus jannaschii	NBRC 100440 = JAL-1 = DSM 2661	Type strain Sequenced genome [DDBJ] The average length is over 10kbp.
103675G	Methanosaeta concilii Synonym: Methanothrix concilii Methanothrix soehngenii	NBRC 103675 = GP6	Type strain
101360G	Methanosaeta thermophila Synonym: Methanothrix thermophila Methanosaeta thermoacetophila Methanothrix thermoacetophila Methanothrix thermoacetophila (sic)	NBRC 101360 = P_T	Type strain Sequenced genome [DDBJ]
100939G	Methanosarcina acetivorans	NBRC 100939 = C2A	Type strain Sequenced genome [DDBJ] The average length is over 10kbp.
100474G	Methanosarcina barkeri	NBRC 100474 = MS	Type strain Reference: Genome sequence of M. barkeri Fusaro [DDBJ]
100827G	Pyrobaculum aerophilum	NBRC 100827 = IM2	Type strain Sequenced genome [DDBJ]
100139G	Pyrococcus horikoshii	NBRC 100139 = OT3	Type strain Sequenced genome [DOGAN] [DDBJ]
15157G	Sulfolobus acidocaldarius	NBRC 15157 = DSM 639	Type strain Sequenced genome [DDBJ]
15437G	Sulfolobus shibatae	NBRC 15437 = B 12	Type strain
100140G	Sulfolobus tokodaii	NBRC 100140 = 7	Type strain Sequenced genome [DOGAN] [DDBJ]
15161G	Sulfurisphaera ohwakuensis	NBRC 15161 = TA 1	Type strain
101555G NEW!!	Thermococcus celericrescens	NBRC 101555 = TS2 = DSM 17994	Type strain The average length is under 10kbp.
15155G	Thermoplasma acidophilum	NBRC 15155 = 122-1B2 =DSM 1728	Type strain Sequenced genome [DDBJ] The minimum quantity is 500ng. The average length is over 10kbp.

Quality of genomic DNAs

The microbial genomic DNA supplied by NBRC is checked by the following quality verification procedure.

i) Quantity: The quantity (over 1µg) is checked by spectrophotometry.
ii) Suitability for enzyme reaction: The suitability for enzyme reaction is checked by the PCR.
iii) Sequencing of the 16S rDNA: The full-region of 16S rDNA is sequenced.
iv) DNA Length: The average length (over 20kbp) is checked by pulse field gel electrophoresis (PFGE).

Ordering Genomic DNAs

1. Distribution Policy

Distribution of the genomic DNA to domestic and foreign institutions for research purposes will be subject to the regulations of Japanese Ministries. We shall ship our microbial genomic DNA to all countries except for those restricted by Japanese government.

2. Ordering Form [download]

After downloading the Ordering Form, please read the attached "Agreement of Biological Resource Transfer and Treatment". If you don't agree with any of the items, we will be unable to accept your order.
Please note both the name(s) of DNA resource(s) and its (their) NBRC number(s) + G in your order, and sign or place a seal in the signature column. (See also the example of description)

3. Inquiry to NBRC

Please fill the order form and send it to the following address by mail or facsimile. Orders by telephone or e-mail are currently not accepted. If your government agency requires a document for importing our genomic DNA resource(s), please send us a necessary form or forms together with the Ordering form. In response to your fax, we will send a proforma invoice by e-mail and start to process your order.

NITE Biological Resource Center (NBRC),
Department of Biotechnology,
National Institute of Technology and Evaluation (NITE)
2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN

FAX: +81-438-52-2329
E-mail: dna@nbrc.nite.go.jp

3. Payment

The price are shown below. Payment via creditcard (VISA or Master) is welcome. We do not accept payment by cash or check.
No DNA resource(s) cannot be shipped until we confirm the receipt of the fee.

4. Shipping

We will ship the requested DNA resource(s) as soon as we confirm remittance of the amount due. We will dispatch the DNA resource(s) as a dried pellet in a plastic tube by Express Mail Service (EMS). FedEx is not available here.

Price List
Public or educational organizations	10,000 JPY / sample
Private companies	20,000 JPY / sample
JPY means Japanese Yen. Not including consumption tax for customers in Japan.

Orders and payment via bank transfer from out side Japan will be charged a bank transfer fee of JPY 6,000.
If you order from an academic or educational institution, please send us a document indicating the fact along with the Ordering Form.

Notice

Depending on the culture, preservation conditions, judicial regulations, etc., DNA resource(s) will be either removed from the distribution list or modified for their distribution conditions without prior notice.
As stated in the "Agreement of Biological Resource Transfer and Treatment" mentioned above, the DNA resource(s) you have received from us should not be transferred to a third party.
We do not disclose your personal information to any third parties. If you have any questions concerning our DNA resources and/or related matters, please send an e-mail to: dna@nbrc.nite.go.jp. We cannot answer to your questions by telephone.

contact us

NITE Biological Resource Center
Department of Biotechnology
National Institute of Technology and Evaluation

JQA-QMA13036

2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN
TEL:+81-438-20-5763
FAX:+81-438-52-2329

Mail:	collection@nbrc.nite.go.jp
MAP:	Wide Area Local Area [Kazusa(Chiba)/Head Office(Tokyo)]