NBRC : Distribution and Deposit of Biological Resources / Human cDNA Clones
Human cDNA Clones
- Human Gateway® Entry Clones [more...]
- Full-length Human cDNA Clones
- Splicing-Variant Human cDNA Clones
|*Gateway® is a registered trademark of Invitrogen.
About Human cDNA Clones
Human cDNA clones were prepared at the Institute of Medical Science of the University of Tokyo (IMS-UT) and Helix Research Institute, Inc (HRI) by the "oligo-capping" method1, 2. And at Kazusa DNA Research Institute (KDRI) the cDNAs were also generated by conventional methods and strictly size-selected.
These clones were provided and analyzed in the following two projects;
NEDO Full-length human cDNA sequencing project
This project was performed by Research Association for Biotechnology (RAB) and supported by the New Energy and Industrial Technology Development Organization (NEDO). It was carried out from 1998 till 2002, in a transition period from the structural to functional genomics. The project has already been completed and resulted in more than 30,000 Full-length human cDNA clones from various human organs. Currently, RAB administers cDNA clones from IMS-UT and HRI independently. The clones were deposited to NBRC and NBRC has started distribution of them since 2002.
NEDO Functional analysis of protein and research application project
"Human cDNA sequencing project" focused on splicing variants of mRNA in this project supported by the Japanese government was carried out from 2002 till 2006 and has resulted in about 25,000 Splicing-varinat human cDNA clones. These clones were deposited to NBRC and NBRC has started the distribution since 2007.
|pME18SFL3 (3,394 bp)||Show Sequence||Word DOC file:35KB|
|pUC19FL3 (3,098 bp)||Show Sequence||Word DOC file:36KB|
Maruyama K, Sugano S, Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides., Gene, 38(1-2), 171-4 (1994) [PubMed: 8125298]
Suzuki et al., Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library., Gene, 200(1-2), 149-56 (1997) [PubMed: 9373149]
Ordering Human cDNA Clones
1. Clone search
Please search the clones of your interest at NBRC Human cDNA clone search system. The system supports Sequence ID Search, Keywords Search and Sequence Search.
|RefSeq Numbers (ex. NM_000000001) are NOT supported by NBRC Human cDNA clone search system since they are not used in DDBJ and EMBL. If you have any questions, please contact us by email.|
2. Material Transfer Agreement (MTA)
Check whether the MTAs are required for the clones of your interest at MTA search system before ordering. If the result at the "Download" column is "MTA is not required", the MTA is not required for distribution of the clones. If MTA is required, please download the MTA by clicking the button shown.
3. Download the Ordering Form [download 179KB]
After downloading the Ordering Form, please read the attached "Agreement of Biological Resource Transfer and Treatment". If you don't agree with any of the items, we will be unable to accept your order.
4. Inquiry to NBRC
If you would agree the items of "Agreement of Biological Resource Transfer and Treatment"of the Ordering Form and the MTA in case of it is required, please complete the following document(s) and send it to the following address by postal mail, facsimile or e-mail. If your government agency requires a document for importing the human Gateway entry clones, please send us a necessary form(s) to us.
AGREEMENT / ORDER FOR NBRC DNA RESOURCES (Ordering Form) [Example ( 37KB)] MTA [if required] (See following Notice.) [Example ( 23KB)]
|Biological Resource Center (NBRC), |
National Institute of Technology and Evaluation
2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN
The signature of the Ordering Form must be handwritten.
If the MTA is required;
a) The name of either the principal investigator of your laboratory, director of the technology transfer office, or an administrative office representative of your organization is required to be entered as the Recipient of the MTAs. His/her signature must be handwritten (a photo-copied signature is not acceptable).
b) You should send TWO original signed MTAs to us by postal mail. They are needed to securely transfer the human cDNA clones and record about it as agreed by the Depositor, NBRC and the Recipient.
To expedite your order, please fax the Ordering Form and MTA to: +81-438-52-2329. In response to your fax, we will send a proforma invoice by e-mail and start to process your order. However, we cannot ship the clone(s) until we confirm the receipt of the fee (see Fee) and the original signed MTAs if they are required.
c) Please fill the column "SCHEDULE 1 MATERIALS" with name(s) of clone(s) which requires the MTA for distribution.
For price list, please click here.
We accept payment by bank remittance or credit card (VISA or Master). In the latter case, please select a check box "credit card" of the Ordering Form. We do not accept payment by cash or check.
- We can ship the requested clones after verifying your payment and receive your two original signed MTAs if they are required.
- We dispatch the clone(s) as a dried DNA pellet in a plastic tube by Express Mail Service (EMS).
Depending on the culture, preservation conditions, judicial regulations, etc., DNA resource(s) will be either removed from the distribution list or modified for their distribution conditions without prior notice.
As stated in the "Agreement of Biological Resource Transfer and Treatment" mentioned above, the DNA resource(s) you have received from us should not be transferred to a third party.
In order to improve our resources, we would like you to indicate the following items in your publication:
- The accession number of human cDNA clone(s); and
- "NBRC, NITE, Kisarazu, Japan" as the source of the clone.
Also, we appreciate receiving reprints of your publications as well as any information concerning your research using the human cDNA clone(s) NBRC has supplied.
We do not disclose your personal information to any third parties. If you have any questions concerning our DNA resources and/or related matters, please send an e-mail to: firstname.lastname@example.org. We cannot answer to your questions by telephone.