NITE Biological Resource Center / Microbial Genomic DNA Clones

Microbial Genomic DNA Clones

NBRC is distributing to both academia and industries copies of the microbial genomic DNA clones constructed during the course of each of the genomic DNA sequencing project. Ordering procedure of the genomic DNA clones is shown below of this page.

Genomic clones of the following microorganisms will be distributed;
Organism	NBRC No.	Database	Comments
Aspergillus oryzae RIB40 [Description]	100959	[DOGAN]	i) See Appendix C. ii) See Appendix D. iii) Clone information is available at DOGAN.
Aeropyrum pernix K1 [Description]	100138	[DOGAN]	i) See Appendix A. ii) The microbial genomic DNA is available.
Pyrococcus horikoshii OT3 [Description]	100139	[DOGAN]	i) See Appendix A. ii) The microbial genomic DNA is available.
Methanocella paludicola SANAE [Description]	101707	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Sulfolobus tokodaii 7 [Description]	100140	[DOGAN]	i) See Appendix A. ii) The microbial genomic DNA is available.
Acetobacter pasteurianusNBRC 105190 (=IFO 3283-32)	105190	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Brevibacillus brevis NBRC 100599 [Description]	100599	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Corynebacterium efficiens YS-314 [Description]	100395	[DOGAN]	i) See Appendix A. ii) The microbial genomic DNA is available.
Deferribacter desulfuricans SSM1 NEW!! [Description]	101012	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Desulfovibrio magneticus RS-1 [Description]	104933	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Gemmatimonas aurantiaca T-27 [Description]	100505	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Kocuria rhizophila DC2201 [Description]	103217	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN. iii) ORF informations were updated on March 31, 2009. Please refer to the DOGANfor details.
Rhodococcus erythropolis PR4 [Description]	100887	[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Rhodococcus opacus B4 [Description]		[DOGAN]	i) See Appendix B. ii) Clone information is available at DOGAN.
Staphylococcus aureus N315 [Description]		[DOGAN]	i) See Appendix A. ii) See Appendix D.
Staphylococcus aureus MW2 [Description]		[DOGAN]	i) See Appendix A. ii) See Appendix D.
Staphylococcus haemolyticus JCSC1435 [Description]		[DOGAN]	i) See Appendix A. ii) See Appendix D.

Appendix A

Since the clones were originally prepared for shot-gun sequencing purpose and the average length of cloned DNA fragments is as short as 1-2 kb, your requested genes/ORFs may possibly be divided into more than one clone. In such a case, please understand that the fee will be multiplied by the number of clones.

Appendix B

The clones were originally prepared as a Fosmid library (about 40kbp) for sequencing purpose.

Appendix C

The clones were originally prepared as a BAC library (aboout 100kbp) for sequencing purpose.

Appendix D

If a clone contains gene/ORF stipulated in Article 48(1) of the Foreign Exchange and Foreign Trade Act, we cannot distribute it to outsite of Japan. For details, please refer to the following documents at the web site of the Japanese Cabinet Secretariat:

Foreign Exchange and Foreign Trade Act
http://www.cas.go.jp/jp/seisaku/hourei/data/FTA.pdf
Foreign Exchange Order
http://www.cas.go.jp/jp/seisaku/hourei/data/FEO.pdf
Export Trade Control Order
http://www.cas.go.jp/jp/seisaku/hourei/data/ETCO.pdf

Ordering Microbial Genomic DNA Clones

1. Distribution Policy

Distribution of the microbial genomic DNA clones to domestic and foreign institutions for research purposes will be subject to the regulations of Japanese Ministries. We shall ship our microbial genomic DNA clones to all countries except for those restricted by Japanese government.

2. Confirm genes/ORFs

You can refer to DOGAN for the clone names of following microoeganisms; A. oryzae RIB40, B. brevis NBRC 100599, D. magnetics RS-1, G. aurantiaca T-27, K. rhizophila DC2201, M. paludicola SANAE, R. erythropolis PR4 and R. opacus B4.
If you are interested in obtaining any clones of other microorganisms listed above, please send the names of the genes/ORFs to: dna@nbrc.nite.go.jp.

3. Reply from NBRC

We shall then inform you of the clones containing your requesting genes/ORFs and associated information by e-mail to which the "AGREEMENT / ORDER FOR NBRC DNA RESOURCES" (The Ordering Form) necessary for clone transfer will be attached. It will take a few days to process your inqury, because we have to check and confirm the clones to meet your request.

4. Fill Ordering Form

Please review the agreement, and if it would be acceptable for you to order the clone(s). Please state "Name of DNA Resources (NBRC No. etc)" of the Ordering Form the clone names exactly. [Example (PDF: 719KB)] The signature of "AGREEMENT / ORDER FOR NBRC DNA RESOURCES"must be handwritten.

3. Inquiry to NBRC

Please send the order form to the following address by mail or facsimile. Orders by telephone or e-mail are currently not accepted. To expedite your order, please fax the Ordering Form at: +81-438-52-2329.
If your government agency requires a document for importing our genomic DNA resource(s), please send us a necessary form or forms together with the Ordering form.

NITE Biological Resource Center (NBRC)
Department of Biotechnology,
National Institute of Technology and Evaluation (NITE)
2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN

3. Payment

The price are shown below. Payment via creditcard (VISA or Master) is welcome. We do not accept payment by cash or check.
No DNA resource(s) cannot be shipped until we confirm the receipt of the fee.

4. Shipping

We will ship the requested DNA resource(s) as soon as we confirm remittance of the amount due. We will dispatch the DNA resource(s) as a dried pellet in a plastic tube by Express Mail Service (EMS). FedEx is not available here.

Price List
Public or educational organizations	4,600 JPY / clone
Private companies	1 - 9 clone(s)	9,200 JPY / clone
Private companies	10 or more clones	8,280 JPY / clone
JPY means Japanese Yen. Not including consumption tax for customers in Japan.

Orders and payment via bank transfer from out side Japan will be charged a bank transfer fee of JPY 6,000.
If you order from an academic or educational institution, please send us a document indicating the fact along with the Ordering Form.

Notice

Depending on the culture, preservation conditions, judicial regulations, etc., DNA resource(s) will be either removed from the distribution list or modified for their distribution conditions without prior notice.
As stated in the "Agreement of Biological Resource Transfer and Treatment" mentioned above, the DNA resource(s) you have received from us should not be transferred to a third party.
We do not disclose your personal information to any third parties. If you have any questions concerning our DNA resources and/or related matters, please send an e-mail to: dna@nbrc.nite.go.jp. We cannot answer to your questions by telephone.

contact us

NITE Biological Resource Center
Department of Biotechnology
National Institute of Technology and Evaluation

JQA-QMA13036

2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN
TEL:+81-438-20-5763
FAX:+81-438-52-2329

Mail:	collection@nbrc.nite.go.jp
MAP:	Wide Area Local Area [Kazusa(Chiba)/Head Office(Tokyo)]