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ISO/JIS/Pharmacopoeia Test Strains
A group of microbial strains authorized by the International Standards (ISO), Japanese Industrial Standards (JIS) and Japanese Pharmacopoeia to be used in officially standardized examinations are included in our collection as listed in Table 1-3. The scientific name of the strains are included in the tables in addition to the names described in each Standard for easier search within our catalogue.
Symbols used in the 'Status' column indicate the status of individual strains in our collection: a double circle ( ) indicates that an NBRC strain is described in the standard, a single circle ( ) indicates that the strains described in the standard is neither an NBRC nor an IFO strains, and hence an NBRC strain sharing the same origin is listed.
| Material |
Strains Designated in the Standard |
NBRC Number |
Status |
ISO 20743:2007(E) Textiles -Determination of antibacterial activity of antibacterial finished products |
7. Reference strains
7.1 Strains |
Staphylococcus aureus |
ATCC 6538, CIP 4.83, DSM 799, NBRC 13276, NCIMB 9518 |
NBRC 13276 |
 |
| Klebsiella pneumoniae |
ATCC 4352, CIP 104216, DSM 789, NBRC 13277, NCIMB 10341 |
NBRC 13277 |
 |
ISO 10705-1:1995(E) Water quality - Detection and enumeration of bacteriophages -- Part 1: Enumeration of F-specific RNA bacteriophages |
 |
Escherichia coli |
K-12 Hfr etc. |
NBRC 106373 |
 |
| Bacteriophage MS2 |
ATCC 15597-B1 etc. |
NBRC 102619 |
 |
ISO 10705-2:2000(E) Water quality - Detection and enumeration of bacteriophages -- Part 2: Enumeration of somatic coliphages |
 |
Escherichia coli |
ATCC 13706 |
NBRC 13898 |
 |
| Bacteriophage φX174 |
ATCC 13706-B1 |
NBRC 103405 |
 |
| Material |
Strains Designated in the Standard |
NBRC Number |
Status |
JIS A 5756:2006 Building gaskets and Building structural gaskets -Materials in prefprmed solid vulcanizates used for sealing glazing and panels |
| 6.13 Anti-fungi test |
Aspergillus terreus |
FERM S-3 |
NBRC 6346 |
 |
| Penicillium pinophilum |
FERM S-6 |
NBRC 6345 |
 |
| Rhizopus oryzae |
FERM S-7 |
NBRC 31005 |
 |
| Cladosporium cladosporioides |
FERM S-8 |
NBRC 6348 |
 |
| Trichoderma virens |
FERM S-10 |
NBRC 6355 |
 |
| Chaetomium globosum |
FERM S-11 |
NBRC 6347 |
 |
| Myrothecium verrucaria |
FERM S-13 |
NBRC 6113 |
 |
JIS B 9918-1:2003 Cleanrooms and associated controlled environments -- Biocontamination control -- Part1: General principles and methods |
 |
Bacillus atrophaeus |
NBRC 14117, ATCC 6633, NCTC 10073, DSM 2277 |
NBRC 14117 |
 |
| Staphylococcus epidermidis |
NBRC 100911, JCM 2414, NCTC 11047, ATCC 14990 |
NBRC 100911 |
 |
| Enterococcus hirae |
NBRC 3181, ATCC 8043 |
NBRC 3181 |
 |
| Saccharomyces cerevisiae |
NBRC 10217, ATCC 18824, JCM 7255 |
NBRC 10217 |
 |
| Aspergillus niger |
NBRC 9455, ATCC 16404 |
NBRC 9455 |
 |
JIS K 1571:2004 Methods for Functional Examination of Wood Preservatives and Their Standards |
| 4.2.1.1.1 Material b) |
A timber fungus
(Fomitopsis palustris) |
FFPRI 0507 |
NBRC 30339 |
 |
A timber fungus
(Trametes versicolor) |
FFPRI 1030 |
NBRC 30340 |
 |
JIS K 3602:2006 Apparatus for the estimation of biochemical oxygen demand (BODs) with microbial sensor |
| 2-(1) |
Trichosporon sp. |
NBRC 10466 |
NBRC 10466 |
 |
JIS K 3703-1:2004 Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)-Part 1:Technique using Baird-Parker agar medium |
| 5. Dilute Solution and Agar Medium --Reference |
coagulase-positive staphylococci
(Staphylococcus aureus subsp. aureus) |
JCM 2151, ATCC 6538P= CCRC 10451, CIP 53.156, DSM 346, FDA 209P, IAM
12082, NBRC 12732, IID 671, KCTC 1927, NCIMB 8625, NCTC 7447, NRIC
1136, NRRL B-313, RIMD 3109007 |
NBRC 12732 |
 |
coagulase-negative staphylococci
(Staphylococcus epidermidis) |
JCM 2414, ATCC 14990, DSM 20044, IAM 12013 |
NBRC 100911 |
 |
JIS K 3703-3:2008 Horizontal method for the enumeration of coagulase--positive staphylococci (Staphylococcus aureus and other species)-Part 3: Detection and MPN technique for low numbers |
| Table 5 |
Staphylococcus aureus |
ATCC 6538, ATCC 6538P, ATCC 25923 etc. |
NBRC 13276
NBRC 12732
NBRC 14462 |
 |
| Escherichia coli |
ATCC 8739, ATCC 25922 etc. |
NBRC 3972
NBRC 15034 |
 |
| Staphylococcus epidermidis |
ATCC 12228 etc. |
NBRC 12993 |
 |
JIS K 3705:2008 Test methods for culture media-Culture medium for Salmonella spp.--Detection of Salmonella spp. |
| Table 6 |
Escherichia coli |
ATCC 8739, ATCC 25922 etc. |
NBRC 3972
NBRC 15034 |
 |
| Enterococcus faecalis |
ATCC 29212, ATCC 19433 etc. |
NBRC 100482
NBRC 100480
NBRC 100481 |
 |
JIS K 3706-1:2008 Test methods for culture media-Culture medium for Listeria monocytogenes--Part 1: Detection of Listeria monocytogenes |
| B.10.4 |
Staphylococcus aureus |
NBRC 11462, NCTC 1803, ATCC 25923 |
NBRC 11462 |
 |
| B. Table 15 |
Escherichia coli |
ATCC 8739, ATCC 25922 etc. |
NBRC 3972
NBRC 15034 |
 |
| Enterococcus faecalis |
ATCC 29212, ATCC 19433 etc. |
NBRC 100482
NBRC 100480
NBRC 100481 |
 |
JIS K 3706-2:2008 Test methods for culture media-Culture medium for Listeria monocytogenes--Part 1: Enumeration of Listeria monocytogenes |
| B.9.4 |
Staphylococcus aureus |
NBRC 11462, NCTC 1803, ATCC 25923 |
NBRC 11462 |
 |
| Rhodococcus equi |
NBRC 14956, NCTC 1621, ATCC 6939 |
NBRC 101255 |
 |
JIS K 3810-1:2006 Methods for the Detection of Mycoplasmas |
| 4. Mycoplasmas for positive control |
Mycoplasma hyorhinis |
ATCC 29052 or NBRC 14858 |
NBRC 14858 |
 |
| Mycoplasma arginini |
ATCC 23838 or NBRC 14476 |
Distribution suspended. |
JIS K 3810-2:2006 Mycoplasma detection methods -- Part 2: Indirect DNA staining assay (Amendment 1) |
| 5.3 Mycoplasmas for positive control |
Mycoplasma hyorhinis |
ATCC 29052 or NBRC 14858 |
NBRC 14858 |
 |
| Mycoplasma arginini |
ATCC 2383 or NBRC 14476 |
Distribution suspended. |
JIS K 3810-3:2006 Mycoplasma detection methods -- Part 3: Nested PCR assay (Amendment 1) |
| 5.3 Detection limit and detection sensitivity of mycoplasma |
Mycoplasma hyorhinis |
ATCC 29052 or NBRC 14858 |
NBRC 14858 |
 |
| Mycoplasma arginini |
ATCC 23838 or NBRC 14476 |
Distribution suspended. |
JIS K 3823:2006 Testing methods for determining bacterial rejection of ultrafiltration modules (Amendment 1) |
| 3.1 Test microorganisms |
Pseudomonas diminuta |
ATCC 19146 or NBRC 14213 |
NBRC 14213 |
 |
JIS K 3835:2006 Testing methods for determining bacterial retention membrane filters (Amendment 1) |
| 3.1 Test microorganisms |
Pseudomonas diminuta |
ATCC 19146 or NBRC 14213 |
NBRC 14213 |
 |
JIS K 3836:2006 Testing methods for collection efficiency of airborne microbe samplers (Amendment 1) |
| 4.1 Microbial particle |
Bacillus subtilis (Bacillus globigii) |
ATCC 9372 or NBRC 13721 |
NBRC 13721 |
 |
JIS K 8008:1992 General rules for biochemical reagents |
| Table 3 |
Bacillus stearothermophilus (Geobacillus stearothermophilus) |
ATCC 7953 |
NBRC 13737 |
 |
Bacillus subtilis var. niger (Bacillus atrophaeus) |
ATCC 9372 |
NBRC 13721
NBRC 16183 |
 |
Pseudomonas diminuta (Brevundimonas diminuta) |
ATCC 19146 |
NBRC 14213 |
 |
Bacillus subtilis var. globigii (Bacillus atrophaeus)) |
NCTC 10073 |
NBRC 14117 |
 |
JIS L 1902:2008 Testing for antibacterial activity and efficacy on textile products |
| 6.1 Bacterial strains |
a) Staphylococcus aureus |
ATCC 6538P, FDA 209P, AHU 1142, IAM 12082, NBRC 12732 etc. |
NBRC 12732 |
 |
| b) Klebsiella pneumoniae |
ATCC 4352, IAM 12015, NBRC 13277 |
NBRC 13277 |
 |
| c) Pseudomonas aeruginosa |
NBRC 3080 |
NBRC 3080 |
 |
| d) Escherichia coli |
NBRC 3301, HUT 8106 |
NBRC 3301 |
 |
JIS R 1702:2006 Fine ceramics (advanced ceramics, advanced technical ceramics) -- Test method for antibacterial activity of photocatalytic products under photoirradiation and efficacy |
| 6. Strains |
Staphylococcus aureus |
ATCC 6538P, DSM 346, NBRC 12732 |
NBRC 12732 |
 |
| Escherichia coli |
ATCC 8739, DSM 1576, NBRC 3972 |
NBRC 3972 |
 |
| Klebsiella pneumoniae |
ATCC 4352, DSM 789, NBRC 13277 |
NBRC 13277 |
 |
JIS R 1705:2010 Fine ceramics (advanced ceramics, advanced technical ceramics) -- Test method for antifungal activity of photocatalytic products under photoirradiation |
| Table 1 |
Aspergillus niger |
NBRC 105649 etc. a) |
NBRC 105649 |
 |
| Penicillium pinophilum |
NBRC 6345 etc. |
NBRC 6345 |
 |
| a) NBRC 6341 designed at version 1 (before 2009) also can be used for this method. |
JIS T 3219:2006 Sterile infusion filter |
| Appendix B |
Brevundimonas diminuta |
NBRC 14213, ATCC 19146 |
NBRC 14213 |
 |
| Serratia marcescens |
Not specified |
NBRC 102204 |
-- |
| Saccharomyces cerevisiae |
Not specified |
NBRC 10217? |
-- |
JIS T 3224:2005 Sterile syringe filter |
| Appendix B |
Brevundimonas diminuta |
NBRC 14213, ATCC 19146 |
NBRC 14213 |
 |
| Serratia marcescens |
Not specified |
NBRC 102204 |
-- |
| Saccharomyces cerevisiae |
Not specified |
NBRC 10217? |
-- |
JIS T 8061:2007 Clothing for protection against contact with blood and body fluids-Determination of resistance of protective clothing materials to penetration by blood-borne pathogens-Test method using Phi-X174 bacteriophage |
 |
Escherichia coliphage φX174 |
ATCC 13706-B1 |
NBRC 103405 |
 |
| Escherichia coli C |
ATCC 13706 |
NBRC 13898 |
 |
JIS W 0812:2004 Airborne equipment-Environmental conditions and test procedures |
| 13.5.2 |
Aspergillus niger |
ATCC 9642 etc. |
NBRC 105650 |
 |
| Aspergillus flavus |
ATCC 9643 etc. |
NBRC 6343 |
 |
| Aspergillus versicolor |
ATCC 11730 etc. |
NBRC 30338 |
 |
| Penicillium funiculosum |
ATCC 11797 etc. |
NBRC 100958 |
 |
| Chaetomium globosum |
ATCC 6205 etc. |
NBRC 6347 |
 |
JIS Z 2801:2000 Antimicrobial products -- Test for antimicrobial activity and efficacy |
| 5.2.1 Strains |
a) Staphylococcus aureus |
ATCC 6538P, FDA 209P, NBRC 12732 |
NBRC 12732 |
 |
| b) Escherichia coli |
ATCC 8739, NBRC 3972 |
NBRC 3972 |
 |
JIS Z 2911:2010 Methods of test for fungus resistance |
| 3. Fungal strains |
1-a) Aspergillus niger |
NBRC 105649 etc.b) |
NBRC 105649 |
 |
| 1-b) Aspergillus niger |
NBRC 105650 etc.c) |
NBRC 105650 |
 |
| 1-c) Eurotium tonophilum |
NBRC 8157, ATCC 16440, IMI 108299ii |
NBRC 8157 |
 |
| 2-a) Penicillium citrinum |
NBRC 6352, ATCC 9849, CBS 342.61, IMI 61272, NRRL 756, QM 1226 |
NBRC 6352 |
 |
| 2-b) Penicillium pinophilum |
NBRC 6345, ATCC 9644, CBS 170.60, IMI 87160ii, NRRL A-5245, QM 391 |
NBRC 6345 |
 |
| 2-b) Penicillium pinophilum |
NBRC 33285 etc. |
NBRC 33285 |
 |
| 3-a) Rhizopus oryzae |
NBRC 31005, ATCC 10404, IMI 61269, QM 387 |
NBRC 31005 |
 |
| 4-a) Cladosporium cladosporioides |
NBRC 6348, IAM F 517 |
NBRC 6348 |
 |
| 4-b) Aureobasidium pullulans |
NBRC 6353, IAM F 24 |
NBRC 6353 |
 |
| 4-c) Trichoderma virens |
NBRC 6355, ATCC 9645, CBS 430.54, IMI 45553ii, QM 365 |
NBRC 6355 |
 |
| 5-a) Chaetomium globosum |
NBRC 6347, ATCC 6205, CBS 148.51, IMI 45550ii, NRRL 1970, QM 459, USDA
1042.4 |
NBRC 6347 |
 |
| 5-b) Myrothecium verrucaria |
NBRC 6113, ATCC 9095, CBS 328.52, NRRL 2003, QM 460, USDA 1334.2 |
NBRC 6113 |
 |
| Appendix B Table B.1 |
Aspergillus terreus |
NBRC 6346, ATCC 10690 |
NBRC 6346 |
 |
| Hormoconis resinae |
NBRC 100535, DSM 1203 |
NBRC 100535 |
 |
| Paecilomyces variotii |
NBRC 30539, ATCC 16023 |
NBRC 30539 |
 |
| Scopulariopsis brevicaulis |
NBRC 100536, ATCC 36840 |
NBRC 100536 |
 |
| Appendix C Table C.1 |
Aspergillus flavus |
NBRC 6343 |
NBRC 6343 |
 |
| Aspergillus flavus |
NBRC 4295 |
NBRC 4295 |
 |
| Aspergillus versicolor |
NBRC 33027 |
NBRC 33027 |
 |
| Paecilomyces variotii |
NBRC 33284 |
NBRC 33284 |
 |
| Aspergillus penicilloides |
NBRC 33024 |
NBRC 33024 |
 |
b) NBRC 6341 designed before 2009 also can be used for this method.
c) NBRC 6342 designed before 2009 also can be used for this method. |
* The 16th version has already been published, but still not English version.
| Methods |
Name of Strain |
Strains described in the Japanese Pharmacopoeia |
NBRC Number |
Status |
General Methods |
4.02 Microbial assay for antibiotics
|
Bacillus subtilis |
ATCC 6633 |
NBRC 3134 |
 |
| Comamonas terrigena |
ATCC 8461 |
NBRC 13299 |
 |
| Escherichia coli |
NIHJ |
NBRC 14237 |
 |
| Klebsiella pneumoniae |
ATCC 10031 |
NBRC 3512 |
 |
Micrococcus luteus
(Kocuria rhizophila) |
ATCC 9341 |
NBRC 12708 |
 |
| Micrococcus luteus |
ATCC 10240 |
NBRC 13867 |
 |
| Penicillium chrysogenum |
ATCC 10002 |
NBRC 4626 |
 |
Proteus sp.
(As cited the sections describing on Fosfomycin-Na and Fosfomycin-Ca.) |
MB 838 |
NBRC 13300 |
 |
| Pseudomonas aeruginosa |
NCTC 10490 |
NBRC 12582 |
 |
| Staphylococcus aureus |
ATCC 6538P |
NBRC 12732 |
 |
| Staphylococcus epidermidis |
ATCC 12228 |
NBRC 12993 |
 |
| 4.05 Microbial limit test |
Aspergillus niger |
NBRC 9455 |
NBRC 9455 |
 |
| Bacillus subtilis |
ATCC 6633, NCIMB 8054, NBRC 3134, JCM 2499 |
NBRC 3134 |
 |
| Candida albicans |
ATCC 2091, ATCC 10231, NBRC 1594, JCM 2085 |
NBRC 1394
NBRC 1594 |
 |
| Clostridium sporogenes |
NBRC 14293 |
NBRC 14293 |
 |
| Escherichia coli |
ATCC 8739, NCIMB 8545, NBRC 3972 |
NBRC 3972 |
 |
| Salmonella enterica subsp. enterica serovar Abony |
NBRC 100797 |
NBRC 100797 |
 |
| Pseudomonas aeruginosa |
ATCC 9027, NCIMB 8626, NBRC 13275
or equivalent strains |
NBRC 13275 |
 |
| Staphylococcus aureus |
ATCC 6538, NCIMB 8625, NBRC 13276
or equivalent strains |
NBRC 13276 |
 |
| 4.06 Sterility test |
Aspergillus niger |
ATCC 16404, NBRC 9455, IP 141.83, IMI 149007 |
NBRC 9455 |
 |
| Bacillus subtilis |
ATCC 6633, NBRC 3134, CIP 52.62, NCIMB 8054 |
NBRC 3134 |
 |
| Candida albicans |
ATCC 10231, NBRC 1594, IP 48.72, NCPF 3179 |
NBRC 1594 |
 |
| Clostridium sporogenes |
ATCC 19404, CIP 79.3, NCTC 532 or ATCC 11437, NBRC 14293 |
NBRC 14293 |
 |
| Pseudomonas aeruginosa |
ATCC 9027, NBRC 13275, NCIMB 8626 |
NBRC 13275 |
 |
| Staphylococcus aureus |
ATCC 6538, NBRC 13276, CIP 4.38, NCTC 10788, NCIMB 9518 |
NBRC 13276 |
 |
5.02 Microbial limit test for crude drugs
1. Total viable aerobic count |
Bacillus subtilis |
NBRC 3134, ATCC 6633, NCIMB 8054 etc. |
NBRC 3134 |
 |
| Candida albicans |
NBRC 1393, NBRC 1594, ATCC 2091, ATCC 10231 etc. |
NBRC 1393
NBRC 1594 |
 |
| Escherichia coli |
NBRC 3972, ATCC 8739, NCIMB 8545
or equivalent strains |
NBRC 3972 |
 |
| Salmonella sp. |
Not specified (non-pathogenic or weekly pathogenic strains are preferred.) |
NBRC 100797 |
 |
| Staphylococcus aureus |
NBRC 13276, ATCC 6538, NCIMB 8625 or equivalent strains |
NBRC 13276 |
 |
General information |
| 14. Mycoplasma testing for cell substrates used for the production of biotechnological/biological products |
M. orale
(Mycoplasma orale) |
CH 19299 |
NBRC 14477 |
 |
| Mycoplasma pneumoniae |
FH |
NBRC 14401 |
 |
| 19. Preservatives-effectiveness tests |
Escherichia coli |
ATCC 8739, NBRC 3972 |
NBRC 3972 |
 |
| Pseudomonas aeruginosa |
ATCC 9027, NBRC 13275 |
NBRC 13275 |
 |
| Staphylococcus aureus |
ATCC 6538, NBRC 13276 |
NBRC 13276 |
 |
| Candida albicans |
ATCC 10231, NBRC 1594, JCM 2085 |
NBRC 1594 |
 |
| Aspergillus niger |
ATCC 16404, NBRC 9455 |
NBRC 9455 |
 |
| 21. Quality control of water for pharmaceutical use |
Escherichia coli |
NBRC 3972 etc. |
NBRC 3972 |
 |
| Pseudomonas aeruginosa |
NBRC 13275 etc. |
NBRC 13275 |
 |
| Staphylococcus aureus |
NBRC 13276 etc. |
NBRC 13276 |
 |
| Pseudomonas fluorescens |
NBRC 15842 etc. |
NBRC 15842 |
 |
| Methylobacterium extorquens |
NBRC 15911 |
NBRC 15911 |
 |
27. Terminal sterilization and sterilization indicators
3. Filteration method |
Brevundimonas diminuta |
ATCC 19146, NBRC 14213, JCM 2428 |
NBRC 14213 |
 |
27. Terminal sterilization and sterilization indicators
4. Sterilization indicators |
Geobacillus stearothermophilus |
ATCC 7953, NBRC 13737, JCM 9488, ATCC 12980, NBRC 12550, JCM 2501 |
NBRC 13737
NBRC 12550 |
 |
| Bacillus atrophaeus |
ATCC 9372, NBRC 13721 |
NBRC 13721 |
 |
|