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ISO/JIS/Pharmacopoeia Test Strains

A group of microbial strains authorized by the International Standards (ISO), Japanese Industrial Standards (JIS) and Japanese Pharmacopoeia to be used in officially standardized examinations are included in our collection as listed in Table 1-3. The scientific name of the strains are included in the tables in addition to the names described in each Standard for easier search within our catalogue.

Symbols used in the 'Status' column indicate the status of individual strains in our collection: a double circle () indicates that an NBRC strain is described in the standard, a single circle () indicates that an IFO strain is described (hence an NBRC strains equivalent to it is listed), while a triangle () indicates that the strains described in the standard is neither an NBRC nor an IFO strains, and hence an equivalent NBRC strain listed.


Table 1. International Standards (ISO)



Material Strains Designated in the Standard NBRC Number Status

ISO 20743:2007(E) Textiles -Determination of antibacterial activity of antibacterial finished products
7. Reference strains
7.1 Strains
Staphylococcus aureus ATCC 6538, CIP 4.83, DSM 799, NBRC 13276, NCIMB 9518 NBRC 13276
Klebsiella pneumoniae ATCC 4352, CIP 104216, DSM 789, NBRC 13277, NCIMB 10341 NBRC 13277


Table 2. Japanese Industrial Standards (JIS)



Material Strains Designated in the Standard NBRC Number Status

JIS K 1571:2004 Methods for Functional Examination of Wood Preservatives and Their Standards
4.2.1.1.1 Material b) A timber fungus
(Fomitopsis palustris)
FFPRI 0507 NBRC 30339
A timber fungus
(Trametes versicolor)
FFPRI 1030 NBRC 30340

JIS K 3810-1:2006 Methods for the Detection of Mycoplasmas
4. Mycoplasmas for positive control Mycoplasma hyorhinis ATCC 29052 or NBRC 14858 NBRC 14858
Mycoplasma arginini ATCC 23838 or NBRC 14476 Distribution suspended.

JIS K 3810-2:2006 Mycoplasma detection methods -- Part 2: Indirect DNA staining assay (Amendment 1)
5.3 Mycoplasmas for positive control Mycoplasma hyorhinis ATCC 29052 or NBRC 14858 NBRC 14858
Mycoplasma arginini ATCC 2383 or NBRC 14476 Distribution suspended.

JIS K 3810-3:2006 Mycoplasma detection methods -- Part 3: Nested PCR assay (Amendment 1)
5.3 Detection limit and detection sensitivity of mycoplasma Mycoplasma hyorhinis ATCC 29052 or NBRC 14858 NBRC 14858
Mycoplasma arginini ATCC 23838 or NBRC 14476 Distribution suspended.

JIS K 3823:2006 Testing methods for determining bacterial rejection of ultrafiltration modules (Amendment 1)
3.1 Test microorganisms Pseudomonas diminuta ATCC 19146 or NBRC 14213 NBRC 14213

JIS K 3835:2006 Testing methods for determining bacterial retention membrane filters (Amendment 1)
3.1 Test microorganisms Pseudomonas diminuta ATCC 19146 or NBRC 14213 NBRC 14213

JIS K 3836:2006 Testing methods for collection efficiency of airborne microbe samplers (Amendment 1)
4.1 Microbial particle Bacillus subtilis
(Bacillus globigii)
ATCC 9372 or NBRC 13721 NBRC 13721

JIS L 1902:2008 Testing for antibacterial activity and efficacy on textile products
6.1 Bacterial strains a) Staphylococcus aureus ATCC 6538P, FDA 209P, AHU 1142, IAM 12082, NBRC 12732 etc. NBRC 12732
b) Klebsiella pneumoniae ATCC 4352, IAM 12015, NBRCO 13277 NBRC 13277
c) Pseudomonas aeruginosa NBRC 3080 NBRC 3080
d) Escherichia coli NBRC 3301, HUT 8106 NBRC 3301

JIS R 1702:2006 Fine ceramics (advanced ceramics, advanced technical ceramics) -- Test method for antibacterial activity of photocatalytic products under photoirradiation and efficacy
6. Strains Staphylococcus aureus ATCC 6538P, DSM 346, NBRC 12732 NBRC 12732
Escherichia coli ATCC 8739, DSM 1576, NBRC 3972 NBRC 3972
Klebsiella pneumoniae ATCC 4352, DSM 789, NBRC 13277 NBRC 13277

JIS Z 2801:2000 Antimicrobial products -- Test for antimicrobial activity and efficacy
5.2.1 Strains a) Staphylococcus aureus ATCC 6538P, FDA 209P, NBRC 12732 NBRC 12732
b) Escherichia coli ATCC 8739, NBRC 3972 NBRC 3972

JIS Z 2911:2000 Methods of test for fungus resistance
3. Fungal strains 1-a) Aspergillus niger NBRC 6341, ATCC 6275, CBS131.52, IMI 45551, NRRL 334, QM 334458, USDA 215-4247 NBRC 6341
1-b) Aspergillus niger NBRC 6342, ATCC 9642, CBS 246.65, IMI 91855, QM 386 NBRC 6342
1-c) Aspergillus terreus NBRC 6346, ATCC 10690, CBS377.64, IMI 45543, NRRL 571, QM 82j NBRC 6346
1-d) Eurotium tonophilum NBRC 8157, ATCC 16440, IMI 108299ii NBRC 8157
2-a) Penicillium citrinum NBRC 6352, ATCC 9849, CBS 342.61, IMI 61272, NRRL 756, QM 1226 NBRC 6352
2-b) Penicillium funiculosum NBRC 6345, ATCC 9644, CBS 170.60, IMI 87160ii, NRRL A-5245, QM 391 NBRC 6345
3-a) Rhizopus oryzae NBRC 31005, ATCC 10404, IMI 61269, QM 387 NBRC 31005
4-a) Cladosporium cladosporioides NBRC 6348, IAM F 517 NBRC 6348
4-b) Aureobasidium pullulans NBRC 6353, IAM F 24 NBRC 6353
4-c) Trichoderma virens NBRC 6355, ATCC 9645, CBS 430.54, IMI 45553ii, QM 365 NBRC 6355
5-a) Chaetomium globosum NBRC 6347, ATCC 6205, CBS 148.51, IMI 45550ii, NRRL 1970, QM 459, USDA 1042.4 NBRC 6347
5-b) Fusarium moniliforme =Gibberella fujikuroi NBRC 6349, ATCC 10052, CBS 263.54, IMI 58292, IMI 61274, QM 1224, USDA 1004.1 NBRC 6349
This strain requires permission of the Plant Protection Station for domestic distribution
5-c) Myrothecium verrucaria NBRC 6113, ATCC 9095, CBS 328.52, NRRL 2003, QM 460, USDA 1334.2 NBRC 6113

Additional Information
JIS K3703-1 (2004)
Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) -- Part 1: Technique using Baird-Parker agar medium
5. Dilute Solution and Agar Medium --Reference coagulase-positive staphylococci
(Staphylococcus aureus subsp. aureus)
JCM 2151 = ATCC 6538P= CCRC 10451 = CIP 53.156 = DSM 346 = FDA 209P = IAM 12082 = NBRC 12732 = IID 671 = KCTC 1927 = NCIMB 8625 = NCTC 7447 = NRIC 1136 = NRRL B-313 = RIMD 3109007 NBRC 12732
coagulase-negative staphylococci
(Staphylococcus epidermidis)
JCM 2414 Type strain = ATCC 14990 = DSM 20044 = IAM 12013 NBRC 100911
Although this strain was not cited, it is a identical strain and available for use.


Table 3. The Japanese Pharmacopoeia



Methods Name of Strain Strains described in the Japanese Pharmacopoeia NBRC Number Status

General Methods
4.02 Activity tests for antibiotics
I . Cylindrical plate method
Staphylococcus aureus ATCC 6538P NBRC 12732
Staphylococcus epidermidis ATCC 12228 NBRC 12993
Micrococcus luteus ATCC 10240 NBRC 13867
Micrococcus luteus
(Kocuria rhizophila)
ATCC 9341 NBRC 12708
Bacillus subtilis ATCC 6633 NBRC 3134
Escherichia coli NIHJ NBRC 14237
Klebsiella pneumoniae ATCC 10031 NBRC 3512
Pseudomonas aeruginosa NCTC 10490 NBRC 12582
Comamonas terrigena ATCC 8461 NBRC 13299
Penicillium chrysogenum ATCC 10002 NBRC 4626
Proteus sp.
(As cited the sections describing on Fosfomycin-Na and Fosfomycin-Ca.)
MB 838 NBRC 13300
4.02 Activity tests for antibiotics
III . Turbidity methods
Staphylococcus aureus ATCC 6538P NBRC 12732
Klebsiella pneumoniae ATCC 10031 NBRC 3512
4.05 Limitation tests for microorganisms
1. Viability tests
Escherichia coli ATCC 8739, NCIMB 8545, NBRC 3972
NBRC 3972
Bacillus subtilis ATCC 6633, NCIMB 8054, NBRC 3134, JCM 2499 NBRC 3134
Staphylococcus aureus ATCC 6538, NCIMB 8625, NBRC 13276 NBRC 13276
Candida albicans ATCC 2091, ATCC 10231, NBRC 1594, JCM 2085 NBRC 1594
4.05 Limitation tests for microorganisms
2. Tests with specified strains
Escherichia coli ATCC 8739, NCIMB 8545, NBRC 3972
or equivalent strains
NBRC 3972
Salmonella sp. Not specified (non-pathogenic or weekly pathogenic strains are preferred. Salmonella Typhi should not be used.) Generally, NBRC 13245 or NBRC 100797 will be distributed.
Pseudomonas aeruginosa ATCC 9027, NCIMB 8626, NBRC 13275
or equivalent strains
NBRC 13275
Staphylococcus aureus ATCC 6538, NCIMB 8625, NBRC 13276
or equivalent strains
NBRC 13276
4.06 Tests for Asepsis
(Performance Tests of Medias and Validation Tests)
Staphylococcus aureus ATCC 6538, NBRC 13276, CIP 4.38, NCTC 10788, NCIMB 9518 NBRC 13276
Pseudomonas aeruginosa ATCC 9027, NBRC 13275, NCIMB 8626 NBRC 13275
Clostridium sporogenes ATCC 19404, CIP 79.3, NCTC 532 or ATCC 11437, NBRC 14293 NBRC 14293
Bacillus subtilis ATCC 6633, NBRC 3134, CIP 52.62, NCIMB 8054 NBRC 3134
Candida albicans ATCC 10231, NBRC 1594, IP 48.72, NCPF 3179 NBRC 1594
Aspergillus niger ATCC 16404, NBRC 9455, IP 141.83, IMI 149007 NBRC 9455
5.02 Limitation tests for natural crude drugs
1. Viability tests
Escherichia coli NBRC 3972, ATCC 8739, NCIMB 8545 etc. NBRC 3972
Bacillus subtilis NBRC 3134, ATCC 6633, NCIMB 8054 etc. NBRC 3134
Staphylococcus aureus NBRC 13276, ATCC 6538, NCIMB 9518 etc. NBRC 13276
Candida albicans NBRC 1393, NBRC 1594, ATCC 2091, ATCC 10231 etc. NBRC 1393
NBRC 1594
Escherichia coli NBRC 3972, ATCC 8739, NCIMB 8545
or equivalent strains
NBRC 3972
Salmonella sp. Not specified (non-pathogenic or weekly pathogenic strains are preferred. Salmonella Typhi should not be used.) Generally, NBRC 13245 or NBRC 100797 will be distributed.
Staphylococcus aureus NBRC 13276, ATCC 6538, NCIMB 8625
or equivalent strains
NBRC 13276

Additional information
11. Ultimate sterilization indicators
3. Filteration method
Brevundimonas diminuta ATCC 19146, NBRC 14213, JCM 2428 NBRC 14213
11. Ultimate sterilization indicators
4. Sterilization indicators
Geobacillus stearothermophilus ATCC 7953, NBRC 13737, JCM 9488, ATCC 12980, NBRC 12550, JCM 2501 NBRC 13737
NBRC 12550
Bacillus atrophaeus ATCC 9372, NBRC 13721 NBRC 13721
20.Negation test for mycoplasma in medical products of applied biotechnology or materials used for medicines originated from living organisms M. orale
(Mycoplasma orale)
CH 19299 NBRC 14477
28. Tests for the effects of preservation Escherichia coli ATCC 8739, NBRC 3972 NBRC 3972
Pseudomonas aeruginosa ATCC 9027, NBRC 13275 NBRC 13275
Staphylococcus aureus ATCC 6538, NBRC 13276 NBRC 13276
Candida albicans ATCC 10231, NBRC 1594, JCM 2085 NBRC 1594
Aspergillus niger ATCC 16404, NBRC 9455 NBRC 9455



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