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ISO/JIS/Pharmacopoeia Test Strains

A group of microbial strains authorized by the International Standards (ISO), Japanese Industrial Standards (JIS) and Japanese Pharmacopoeia to be used in officially standardized examinations are included in our collection as listed in Table 1-3. The scientific name of the strains are included in the tables in addition to the names described in each Standard for easier search within our catalogue.

Symbols used in the 'Status' column indicate the status of individual strains in our collection: a double circle () indicates that an NBRC strain is described in the standard, a single circle () indicates that the strains described in the standard is neither an NBRC nor an IFO strains, and hence an NBRC strain sharing the same origin is listed.


Table 1. International Standards (ISO)



Material Strains Designated in the Standard NBRC Number Status

ISO 20743:2007(E) Textiles -Determination of antibacterial activity of antibacterial finished products
7. Reference strains
7.1 Strains
Staphylococcus aureus ATCC 6538, CIP 4.83, DSM 799, NBRC 13276, NCIMB 9518 NBRC 13276
Klebsiella pneumoniae ATCC 4352, CIP 104216, DSM 789, NBRC 13277, NCIMB 10341 NBRC 13277

ISO 10705-1:1995(E) Water quality - Detection and enumeration of bacteriophages -- Part 1: Enumeration of F-specific RNA bacteriophages
Escherichia coli K-12 Hfr etc. NBRC 106373
Bacteriophage MS2 ATCC 15597-B1 etc. NBRC 102619

ISO 10705-2:2000(E) Water quality - Detection and enumeration of bacteriophages -- Part 2: Enumeration of somatic coliphages
Escherichia coli ATCC 13706 NBRC 13898
Bacteriophage φX174 ATCC 13706-B1 NBRC 103405


Table 2. Japanese Industrial Standards (JIS)



Material Strains Designated in the Standard NBRC Number Status

JIS A 5756:2006 Building gaskets and Building structural gaskets -Materials in prefprmed solid vulcanizates used for sealing glazing and panels
6.13 Anti-fungi test Aspergillus terreus FERM S-3 NBRC 6346
Penicillium pinophilum FERM S-6 NBRC 6345
Rhizopus oryzae FERM S-7 NBRC 31005
Cladosporium cladosporioides FERM S-8 NBRC 6348
Trichoderma virens FERM S-10 NBRC 6355
Chaetomium globosum FERM S-11 NBRC 6347
Myrothecium verrucaria FERM S-13 NBRC 6113

JIS B 9918-1:2003 Cleanrooms and associated controlled environments -- Biocontamination control -- Part1: General principles and methods
Bacillus atrophaeus NBRC 14117, ATCC 6633, NCTC 10073, DSM 2277 NBRC 14117
Staphylococcus epidermidis NBRC 100911, JCM 2414, NCTC 11047, ATCC 14990 NBRC 100911
Enterococcus hirae NBRC 3181, ATCC 8043 NBRC 3181
Saccharomyces cerevisiae NBRC 10217, ATCC 18824, JCM 7255 NBRC 10217
Aspergillus niger NBRC 9455, ATCC 16404 NBRC 9455

JIS K 1571:2004 Methods for Functional Examination of Wood Preservatives and Their Standards
4.2.1.1.1 Material b) A timber fungus
(Fomitopsis palustris)
FFPRI 0507 NBRC 30339
A timber fungus
(Trametes versicolor)
FFPRI 1030 NBRC 30340

JIS K 3602:2006 Apparatus for the estimation of biochemical oxygen demand (BODs) with microbial sensor
2-(1) Trichosporon sp. NBRC 10466 NBRC 10466

JIS K 3703-1:2004 Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)-Part 1:Technique using Baird-Parker agar medium
5. Dilute Solution and Agar Medium --Reference coagulase-positive staphylococci
(Staphylococcus aureus subsp. aureus)
JCM 2151, ATCC 6538P= CCRC 10451, CIP 53.156, DSM 346, FDA 209P, IAM 12082, NBRC 12732, IID 671, KCTC 1927, NCIMB 8625, NCTC 7447, NRIC 1136, NRRL B-313, RIMD 3109007 NBRC 12732
coagulase-negative staphylococci
(Staphylococcus epidermidis)
JCM 2414, ATCC 14990, DSM 20044, IAM 12013 NBRC 100911

JIS K 3703-3:2008 Horizontal method for the enumeration of coagulase--positive staphylococci (Staphylococcus aureus and other species)-Part 3: Detection and MPN technique for low numbers
Table 5 Staphylococcus aureus ATCC 6538, ATCC 6538P, ATCC 25923 etc. NBRC 13276
NBRC 12732
NBRC 14462
Escherichia coli ATCC 8739, ATCC 25922 etc. NBRC 3972
NBRC 15034
Staphylococcus epidermidis ATCC 12228 etc. NBRC 12993

JIS K 3705:2008 Test methods for culture media-Culture medium for Salmonella spp.--Detection of Salmonella spp.
Table 6 Escherichia coli ATCC 8739, ATCC 25922 etc. NBRC 3972
NBRC 15034
Enterococcus faecalis ATCC 29212, ATCC 19433 etc. NBRC 100482
NBRC 100480
NBRC 100481

JIS K 3706-1:2008 Test methods for culture media-Culture medium for Listeria monocytogenes--Part 1: Detection of Listeria monocytogenes
B.10.4 Staphylococcus aureus NBRC 11462, NCTC 1803, ATCC 25923 NBRC 11462
B. Table 15 Escherichia coli ATCC 8739, ATCC 25922 etc. NBRC 3972
NBRC 15034
Enterococcus faecalis ATCC 29212, ATCC 19433 etc. NBRC 100482
NBRC 100480
NBRC 100481

JIS K 3706-2:2008 Test methods for culture media-Culture medium for Listeria monocytogenes--Part 1: Enumeration of Listeria monocytogenes
B.9.4 Staphylococcus aureus NBRC 11462, NCTC 1803, ATCC 25923 NBRC 11462
Rhodococcus equi NBRC 14956, NCTC 1621, ATCC 6939 NBRC 101255

JIS K 3810-1:2006 Methods for the Detection of Mycoplasmas
4. Mycoplasmas for positive control Mycoplasma hyorhinis ATCC 29052 or NBRC 14858 NBRC 14858
Mycoplasma arginini ATCC 23838 or NBRC 14476 Distribution suspended.

JIS K 3810-2:2006 Mycoplasma detection methods -- Part 2: Indirect DNA staining assay (Amendment 1)
5.3 Mycoplasmas for positive control Mycoplasma hyorhinis ATCC 29052 or NBRC 14858 NBRC 14858
Mycoplasma arginini ATCC 2383 or NBRC 14476 Distribution suspended.

JIS K 3810-3:2006 Mycoplasma detection methods -- Part 3: Nested PCR assay (Amendment 1)
5.3 Detection limit and detection sensitivity of mycoplasma Mycoplasma hyorhinis ATCC 29052 or NBRC 14858 NBRC 14858
Mycoplasma arginini ATCC 23838 or NBRC 14476 Distribution suspended.

JIS K 3823:2006 Testing methods for determining bacterial rejection of ultrafiltration modules (Amendment 1)
3.1 Test microorganisms Pseudomonas diminuta ATCC 19146 or NBRC 14213 NBRC 14213

JIS K 3835:2006 Testing methods for determining bacterial retention membrane filters (Amendment 1)
3.1 Test microorganisms Pseudomonas diminuta ATCC 19146 or NBRC 14213 NBRC 14213

JIS K 3836:2006 Testing methods for collection efficiency of airborne microbe samplers (Amendment 1)
4.1 Microbial particle Bacillus subtilis
(Bacillus globigii)
ATCC 9372 or NBRC 13721 NBRC 13721

JIS K 8008:1992 General rules for biochemical reagents
Table 3 Bacillus stearothermophilus
(Geobacillus stearothermophilus)
ATCC 7953 NBRC 13737
Bacillus subtilis var. niger
(Bacillus atrophaeus)
ATCC 9372 NBRC 13721
NBRC 16183
Pseudomonas diminuta
(Brevundimonas diminuta)
ATCC 19146 NBRC 14213
Bacillus subtilis var. globigii
(Bacillus atrophaeus))
NCTC 10073 NBRC 14117

JIS L 1902:2008 Testing for antibacterial activity and efficacy on textile products
6.1 Bacterial strains a) Staphylococcus aureus ATCC 6538P, FDA 209P, AHU 1142, IAM 12082, NBRC 12732 etc. NBRC 12732
b) Klebsiella pneumoniae ATCC 4352, IAM 12015, NBRC 13277 NBRC 13277
c) Pseudomonas aeruginosa NBRC 3080 NBRC 3080
d) Escherichia coli NBRC 3301, HUT 8106 NBRC 3301

JIS R 1702:2006 Fine ceramics (advanced ceramics, advanced technical ceramics) -- Test method for antibacterial activity of photocatalytic products under photoirradiation and efficacy
6. Strains Staphylococcus aureus ATCC 6538P, DSM 346, NBRC 12732 NBRC 12732
Escherichia coli ATCC 8739, DSM 1576, NBRC 3972 NBRC 3972
Klebsiella pneumoniae ATCC 4352, DSM 789, NBRC 13277 NBRC 13277

JIS R 1705:2010 Fine ceramics (advanced ceramics, advanced technical ceramics) -- Test method for antifungal activity of photocatalytic products under photoirradiation
Table 1 Aspergillus niger NBRC 105649 etc. a) NBRC 105649
Penicillium pinophilum NBRC 6345 etc. NBRC 6345
a) NBRC 6341 designed at version 1 (before 2009) also can be used for this method.

JIS T 3219:2006 Sterile infusion filter
Appendix B Brevundimonas diminuta NBRC 14213, ATCC 19146 NBRC 14213
Serratia marcescens Not specified NBRC 102204 --
Saccharomyces cerevisiae Not specified NBRC 10217? --

JIS T 3224:2005 Sterile syringe filter
Appendix B Brevundimonas diminuta NBRC 14213, ATCC 19146 NBRC 14213
Serratia marcescens Not specified NBRC 102204 --
Saccharomyces cerevisiae Not specified NBRC 10217? --

JIS T 8061:2007 Clothing for protection against contact with blood and body fluids-Determination of resistance of protective clothing materials to penetration by blood-borne pathogens-Test method using Phi-X174 bacteriophage
Escherichia coliphage φX174 ATCC 13706-B1 NBRC 103405
Escherichia coli C ATCC 13706 NBRC 13898

JIS W 0812:2004 Airborne equipment-Environmental conditions and test procedures
13.5.2 Aspergillus niger ATCC 9642 etc. NBRC 105650
Aspergillus flavus ATCC 9643 etc. NBRC 6343
Aspergillus versicolor ATCC 11730 etc. NBRC 30338
Penicillium funiculosum ATCC 11797 etc. NBRC 100958
Chaetomium globosum ATCC 6205 etc. NBRC 6347

JIS Z 2801:2000 Antimicrobial products -- Test for antimicrobial activity and efficacy
5.2.1 Strains a) Staphylococcus aureus ATCC 6538P, FDA 209P, NBRC 12732 NBRC 12732
b) Escherichia coli ATCC 8739, NBRC 3972 NBRC 3972

JIS Z 2911:2010 Methods of test for fungus resistance
3. Fungal strains 1-a) Aspergillus niger NBRC 105649 etc.b) NBRC 105649
1-b) Aspergillus niger NBRC 105650 etc.c) NBRC 105650
1-c) Eurotium tonophilum NBRC 8157, ATCC 16440, IMI 108299ii NBRC 8157
2-a) Penicillium citrinum NBRC 6352, ATCC 9849, CBS 342.61, IMI 61272, NRRL 756, QM 1226 NBRC 6352
2-b) Penicillium pinophilum NBRC 6345, ATCC 9644, CBS 170.60, IMI 87160ii, NRRL A-5245, QM 391 NBRC 6345
2-b) Penicillium pinophilum NBRC 33285 etc. NBRC 33285
3-a) Rhizopus oryzae NBRC 31005, ATCC 10404, IMI 61269, QM 387 NBRC 31005
4-a) Cladosporium cladosporioides NBRC 6348, IAM F 517 NBRC 6348
4-b) Aureobasidium pullulans NBRC 6353, IAM F 24 NBRC 6353
4-c) Trichoderma virens NBRC 6355, ATCC 9645, CBS 430.54, IMI 45553ii, QM 365 NBRC 6355
5-a) Chaetomium globosum NBRC 6347, ATCC 6205, CBS 148.51, IMI 45550ii, NRRL 1970, QM 459, USDA 1042.4 NBRC 6347
5-b) Myrothecium verrucaria NBRC 6113, ATCC 9095, CBS 328.52, NRRL 2003, QM 460, USDA 1334.2 NBRC 6113
Appendix B Table B.1 Aspergillus terreus NBRC 6346, ATCC 10690 NBRC 6346
Hormoconis resinae NBRC 100535, DSM 1203 NBRC 100535
Paecilomyces variotii NBRC 30539, ATCC 16023 NBRC 30539
Scopulariopsis brevicaulis NBRC 100536, ATCC 36840 NBRC 100536
Appendix C Table C.1 Aspergillus flavus NBRC 6343 NBRC 6343
Aspergillus flavus NBRC 4295 NBRC 4295
Aspergillus versicolor NBRC 33027 NBRC 33027
Paecilomyces variotii NBRC 33284 NBRC 33284
Aspergillus penicilloides NBRC 33024 NBRC 33024
b) NBRC 6341 designed before 2009 also can be used for this method.
c) NBRC 6342 designed before 2009 also can be used for this method.


Table 3. The Japanese Pharmacopoeia (15th edition*)

* The 16th version has already been published, but still not English version.

Methods Name of Strain Strains described in the Japanese Pharmacopoeia NBRC Number Status

General Methods
4.02 Microbial assay for antibiotics
Bacillus subtilis ATCC 6633 NBRC 3134
Comamonas terrigena ATCC 8461 NBRC 13299
Escherichia coli NIHJ NBRC 14237
Klebsiella pneumoniae ATCC 10031 NBRC 3512
Micrococcus luteus
(Kocuria rhizophila)
ATCC 9341 NBRC 12708
Micrococcus luteus ATCC 10240 NBRC 13867
Penicillium chrysogenum ATCC 10002 NBRC 4626
Proteus sp.
(As cited the sections describing on Fosfomycin-Na and Fosfomycin-Ca.)
MB 838 NBRC 13300
Pseudomonas aeruginosa NCTC 10490 NBRC 12582
Staphylococcus aureus ATCC 6538P NBRC 12732
Staphylococcus epidermidis ATCC 12228 NBRC 12993
4.05 Microbial limit test Aspergillus niger NBRC 9455 NBRC 9455
Bacillus subtilis ATCC 6633, NCIMB 8054, NBRC 3134, JCM 2499 NBRC 3134
Candida albicans ATCC 2091, ATCC 10231, NBRC 1594, JCM 2085 NBRC 1394
NBRC 1594
Clostridium sporogenes NBRC 14293 NBRC 14293
Escherichia coli ATCC 8739, NCIMB 8545, NBRC 3972 NBRC 3972
Salmonella enterica subsp. enterica serovar Abony NBRC 100797 NBRC 100797
Pseudomonas aeruginosa ATCC 9027, NCIMB 8626, NBRC 13275
or equivalent strains
NBRC 13275
Staphylococcus aureus ATCC 6538, NCIMB 8625, NBRC 13276
or equivalent strains
NBRC 13276
4.06 Sterility test Aspergillus niger ATCC 16404, NBRC 9455, IP 141.83, IMI 149007 NBRC 9455
Bacillus subtilis ATCC 6633, NBRC 3134, CIP 52.62, NCIMB 8054 NBRC 3134
Candida albicans ATCC 10231, NBRC 1594, IP 48.72, NCPF 3179 NBRC 1594
Clostridium sporogenes ATCC 19404, CIP 79.3, NCTC 532 or ATCC 11437, NBRC 14293 NBRC 14293
Pseudomonas aeruginosa ATCC 9027, NBRC 13275, NCIMB 8626 NBRC 13275
Staphylococcus aureus ATCC 6538, NBRC 13276, CIP 4.38, NCTC 10788, NCIMB 9518 NBRC 13276
5.02 Microbial limit test for crude drugs
1. Total viable aerobic count
Bacillus subtilis NBRC 3134, ATCC 6633, NCIMB 8054 etc. NBRC 3134
Candida albicans NBRC 1393, NBRC 1594, ATCC 2091, ATCC 10231 etc. NBRC 1393
NBRC 1594
Escherichia coli NBRC 3972, ATCC 8739, NCIMB 8545
or equivalent strains
NBRC 3972
Salmonella sp. Not specified (non-pathogenic or weekly pathogenic strains are preferred.) NBRC 100797
Staphylococcus aureus NBRC 13276, ATCC 6538, NCIMB 8625
or equivalent strains
NBRC 13276

General information
14. Mycoplasma testing for cell substrates used for the production of biotechnological/biological products M. orale
(Mycoplasma orale)
CH 19299 NBRC 14477
Mycoplasma pneumoniae FH NBRC 14401
19. Preservatives-effectiveness tests Escherichia coli ATCC 8739, NBRC 3972 NBRC 3972
Pseudomonas aeruginosa ATCC 9027, NBRC 13275 NBRC 13275
Staphylococcus aureus ATCC 6538, NBRC 13276 NBRC 13276
Candida albicans ATCC 10231, NBRC 1594, JCM 2085 NBRC 1594
Aspergillus niger ATCC 16404, NBRC 9455 NBRC 9455
21. Quality control of water for pharmaceutical use Escherichia coli NBRC 3972 etc. NBRC 3972
Pseudomonas aeruginosa NBRC 13275 etc. NBRC 13275
Staphylococcus aureus NBRC 13276 etc. NBRC 13276
Pseudomonas fluorescens NBRC 15842 etc. NBRC 15842
Methylobacterium extorquens NBRC 15911 NBRC 15911
27. Terminal sterilization and sterilization indicators
3. Filteration method
Brevundimonas diminuta ATCC 19146, NBRC 14213, JCM 2428 NBRC 14213
27. Terminal sterilization and sterilization indicators
4. Sterilization indicators
Geobacillus stearothermophilus ATCC 7953, NBRC 13737, JCM 9488, ATCC 12980, NBRC 12550, JCM 2501 NBRC 13737
NBRC 12550
Bacillus atrophaeus ATCC 9372, NBRC 13721 NBRC 13721



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